| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Frankia | CCACCGGTACAGCCACATT | TCGACAGTTCCAGCGTGAC | 60.00 | 60.01 | 260 | 32 |
100.00%
|
50.00%
|
| Primer 22 | Frankia | TGACCATGCCGTTCGGAT | CGCAGGTTCTCGAACTCGT | 59.01 | 60.08 | 226 | 12 |
100.00%
|
50.00%
|
| Primer 23 | Frankia | TGCATCGTCATCTCGGTGG | ATCTCCAGGTCGTAGCCGT | 59.86 | 60.08 | 200 | 12 |
100.00%
|
50.00%
|