| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Gordonibacter | TGGAGAAGCGGATGGAAGC | TGGCTATGGCATCCCACAG | 59.78 | 59.47 | 225 | 132 |
100.00%
|
66.67%
|
| Primer 2 | Gordonibacter | TGGAGAAGCGGATGGAAGC | TATGGCATCCCACAGCTCC | 59.78 | 59.16 | 221 | 132 |
100.00%
|
66.67%
|
| Primer 3 | Gordonibacter | ACAGTGGAACGGCATCGAG | TGCAGCAGTCGCACTTGT | 60.08 | 60.20 | 290 | 128 |
100.00%
|
66.67%
|
| Primer 4 | Gordonibacter | TGCGAGATGGCCTGCAAA | TCTCGGCATCCTTGTGCA | 59.97 | 58.93 | 214 | 127 |
100.00%
|
100.00%
|
| Primer 5 | Gordonibacter | TGCGAGATGGCCTGCAAA | GTCTCGGCATCCTTGTGCA | 59.97 | 60.38 | 215 | 127 |
100.00%
|
100.00%
|
| Primer 6 | Gordonibacter | TGCGAGATGGCCTGCAAA | TCTCGGCATCCTTGTGCAT | 59.97 | 59.40 | 214 | 127 |
100.00%
|
100.00%
|
| Primer 7 | Gordonibacter | TCGCGTCATCGTCAAGCA | TTGTCGCCTTCCTTGACCG | 59.74 | 60.30 | 168 | 125 |
100.00%
|
100.00%
|
| Primer 8 | Gordonibacter | TGGAGAAGCGGATGGAAGC | TGGCTATCACCACCAGCAC | 59.78 | 59.70 | 168 | 125 |
100.00%
|
66.67%
|
| Primer 9 | Gordonibacter | CTGCAAACAGTGGAACGGC | TGCAGCAGTCGCACTTGT | 60.01 | 60.20 | 296 | 123 |
100.00%
|
100.00%
|
| Primer 10 | Gordonibacter | TGCAAACAGTGGAACGGC | TGCAGCAGTCGCACTTGT | 58.88 | 60.20 | 295 | 123 |
100.00%
|
66.67%
|