| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1421 | Gracilibacillus | AAACAGCGCTGCCTTGCA | AAGCGTGTGGGTGGCATT | 61.22 | 60.20 | 190 | 90 |
100.00%
|
50.00%
|
| Primer 1422 | Gracilibacillus | CGCCTCAAGAAATGGAAGCG | GCTGTTGCAATTGTTGACGT | 59.90 | 58.16 | 168 | 90 |
100.00%
|
50.00%
|
| Primer 1423 | Gracilibacillus | ACCGCTCGCTGTAGCAAA | AAGGCACGCTCCATTCGT | 59.66 | 59.65 | 180 | 90 |
100.00%
|
50.00%
|
| Primer 1424 | Gracilibacillus | ACAAGCCGTGCAAAGAGC | AGGGAACTTCCATCTGCCAT | 58.97 | 58.70 | 267 | 90 |
100.00%
|
50.00%
|
| Primer 1425 | Gracilibacillus | GGAAGCAGGAGTTGGCACA | CGTTTTGGGCAGGGAAAGC | 60.23 | 60.01 | 178 | 90 |
100.00%
|
50.00%
|
| Primer 1426 | Gracilibacillus | GGCAACCATGTTCCGCAT | AGCTTCCAACCTCGTCTGC | 58.71 | 60.00 | 262 | 90 |
100.00%
|
50.00%
|
| Primer 1427 | Gracilibacillus | GGCAACCATGTTCCGCAT | AACCTCGTCTGCAGCCAT | 58.71 | 58.93 | 255 | 90 |
100.00%
|
50.00%
|
| Primer 1428 | Gracilibacillus | TGGTCTCTCCTCCTCAGTGG | TGATCCCCTGCCACGAAA | 59.96 | 58.51 | 194 | 90 |
100.00%
|
50.00%
|
| Primer 1429 | Gracilibacillus | AGACGCGTACGCATGAACA | ACACCAATCCGCTTCGCA | 60.08 | 59.97 | 181 | 90 |
100.00%
|
50.00%
|
| Primer 1430 | Gracilibacillus | GACGCGTACGCATGAACA | ACACCAATCCGCTTCGCA | 58.55 | 59.97 | 180 | 90 |
100.00%
|
50.00%
|