| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 251 | Gracilibacillus | TTTGATCGGTGCTGGCCT | CCTCACCTTGAAAGAAGCGC | 59.24 | 59.48 | 266 | 122 |
100.00%
|
50.00%
|
| Primer 252 | Gracilibacillus | GTGCAAGGTTGGTGGGCTA | GTGCTCACGGTTCCACTGA | 60.23 | 59.93 | 188 | 122 |
100.00%
|
50.00%
|
| Primer 253 | Gracilibacillus | TGCAAGGTTGGTGGGCTA | GTGCTCACGGTTCCACTGA | 58.74 | 59.93 | 187 | 122 |
100.00%
|
50.00%
|
| Primer 254 | Gracilibacillus | ATCCAACTCGCGAGGAAGG | ATGCTTGACTCCCACGTGG | 59.48 | 60.00 | 228 | 122 |
100.00%
|
50.00%
|
| Primer 255 | Gracilibacillus | CGAAGTGTGGGACGTGTCA | ACCAACAGTAAGCGGTCGTT | 59.93 | 59.89 | 275 | 122 |
100.00%
|
50.00%
|
| Primer 256 | Gracilibacillus | TTTTCACCGGTGCTGGCA | ACCACCAAACCAAGCACCT | 60.12 | 59.69 | 252 | 122 |
100.00%
|
50.00%
|
| Primer 257 | Gracilibacillus | TGCTCCTTCGCTTAGCAGG | AAGATCGTTGCTTTACCGGTT | 59.78 | 58.23 | 226 | 122 |
100.00%
|
50.00%
|
| Primer 258 | Gracilibacillus | GCTCCTTCGCTTAGCAGGA | AAGATCGTTGCTTTACCGGTT | 59.48 | 58.23 | 225 | 122 |
100.00%
|
50.00%
|
| Primer 259 | Gracilibacillus | GCTCCTTCGCTTAGCAGGAA | AAGATCGTTGCTTTACCGGTT | 60.11 | 58.23 | 225 | 122 |
100.00%
|
50.00%
|
| Primer 260 | Gracilibacillus | AGGTACAATGCTCCTTCGCT | AAGATCGTTGCTTTACCGGTT | 59.09 | 58.23 | 234 | 122 |
100.00%
|
50.00%
|