| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4081 | Gracilibacillus | TGTCGTGGCGATTGCAGT | AGCTCCATCTATATCCGCTGC | 59.97 | 59.52 | 244 | 65 |
100.00%
|
50.00%
|
| Primer 4082 | Gracilibacillus | TGCGATTGGAGGCAGTGA | CGCCAATAACGACTGCAGC | 58.93 | 59.94 | 194 | 65 |
100.00%
|
50.00%
|
| Primer 4083 | Gracilibacillus | TTGCAGAGCCCAGCTTCA | GCGGAATCGGCACAATTGT | 59.16 | 59.49 | 290 | 65 |
100.00%
|
50.00%
|
| Primer 4084 | Gracilibacillus | TTGCAGAGCCCAGCTTCA | TCGTTAGCGGAATCGGCAC | 59.16 | 60.52 | 296 | 65 |
100.00%
|
50.00%
|
| Primer 4085 | Gracilibacillus | CGAAAGCAAGAAATGGTGCGA | AAGGGGTTCAGGTCTGGCT | 60.07 | 60.46 | 159 | 65 |
100.00%
|
50.00%
|
| Primer 4086 | Gracilibacillus | GAGGGTTTCCAGCCACAGT | CGTTGTTGTTGCCCACCAG | 59.54 | 59.93 | 178 | 65 |
100.00%
|
50.00%
|
| Primer 4087 | Gracilibacillus | TGAAGGGGAAGGTTGTTCGA | TCGCGACTGCCTTTTCCAT | 58.87 | 60.00 | 245 | 65 |
100.00%
|
50.00%
|
| Primer 4088 | Gracilibacillus | TGAAGGGGAAGGTTGTTCGA | TGCTCATCAGTCGCGACT | 58.87 | 58.72 | 255 | 65 |
100.00%
|
50.00%
|
| Primer 4089 | Gracilibacillus | GGCAGAGGAAAACAAACTCGA | TTCGTCTTGTGCTGCGCT | 58.78 | 60.28 | 274 | 65 |
100.00%
|
50.00%
|
| Primer 4090 | Gracilibacillus | GGCAGAGGAAAACAAACTCGA | TTTCGTCTTGTGCTGCGC | 58.78 | 59.37 | 275 | 65 |
100.00%
|
50.00%
|