| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4151 | Gracilibacillus | ATCCAGGCCTCCAAGAACA | CGTGCGAAGGTTCCACTGA | 58.21 | 60.30 | 186 | 65 |
100.00%
|
50.00%
|
| Primer 4152 | Gracilibacillus | TGCCGACACGAACGAATGT | ACCAGTACCACCGTGGAGT | 60.30 | 60.15 | 175 | 65 |
100.00%
|
50.00%
|
| Primer 4153 | Gracilibacillus | AAGGGATCGAACCTATGGGG | CTGTGTCAAAGTCATCGTCGA | 58.56 | 58.34 | 286 | 65 |
100.00%
|
50.00%
|
| Primer 4154 | Gracilibacillus | GGCAGTTGCACTAGCGGTA | ACTTCAAGCCCTGGTCGA | 60.08 | 58.11 | 171 | 65 |
100.00%
|
50.00%
|
| Primer 4155 | Gracilibacillus | TGTGGTGGAAACGGCTGA | TGGTCCGTTGATTGCGTTG | 59.09 | 59.05 | 191 | 65 |
100.00%
|
50.00%
|
| Primer 4156 | Gracilibacillus | ACGAGAGCAAGCAACGGA | TCTGGCATTTCCGCACGA | 59.27 | 59.65 | 234 | 65 |
100.00%
|
50.00%
|
| Primer 4157 | Gracilibacillus | TGCGATTAAGCACACTGGGA | ACCGCAGCCAACATCATGA | 59.68 | 60.00 | 274 | 65 |
100.00%
|
50.00%
|
| Primer 4158 | Gracilibacillus | AGCTGGCAGTTTACCGCA | ACCGTCTTTCGCCATCGA | 59.57 | 59.03 | 277 | 65 |
100.00%
|
50.00%
|
| Primer 4159 | Gracilibacillus | TTGACGAGTTGGCTGAACGA | TCTCCGGTGGTCTCATCGT | 59.90 | 60.00 | 214 | 65 |
100.00%
|
50.00%
|
| Primer 4160 | Gracilibacillus | TCACCTCTGAGAAATTTGCCG | ACCAACCGCATCAGCTGA | 58.57 | 59.25 | 172 | 65 |
100.00%
|
50.00%
|