| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4421 | Gracilibacillus | TCGGCAGAGTGTGAGCAA | ATCGCCACCCCAATTGGT | 58.86 | 59.23 | 272 | 63 |
100.00%
|
50.00%
|
| Primer 4422 | Gracilibacillus | TTCGGCAGAGTGTGAGCA | ATCGCCACCCCAATTGGT | 58.86 | 59.23 | 273 | 63 |
100.00%
|
50.00%
|
| Primer 4423 | Gracilibacillus | ACCGCTGAGGCAAAAGGA | TGCTACGACCTCTGCTCCT | 59.16 | 60.00 | 288 | 63 |
100.00%
|
50.00%
|
| Primer 4424 | Gracilibacillus | AACAGGGCAAGCAGTCGAA | GGACACCAGGTTGCCCAAA | 59.85 | 60.46 | 240 | 63 |
100.00%
|
50.00%
|
| Primer 4425 | Gracilibacillus | TGTTTCCGTGGCCTTGGA | TGGAGCGTTGGATTGGCT | 59.08 | 59.24 | 275 | 63 |
100.00%
|
50.00%
|
| Primer 4426 | Gracilibacillus | TGGTGTCCTGCAGTTGCT | ACCGCTTGATGCTGCCTA | 59.08 | 59.01 | 165 | 63 |
100.00%
|
50.00%
|
| Primer 4427 | Gracilibacillus | TGGAGGAGCCTCAAGTGGT | CAGCAAGCTGATCGGCAA | 60.15 | 58.42 | 185 | 63 |
100.00%
|
50.00%
|
| Primer 4428 | Gracilibacillus | TCGTGCGATGAATTATGTGCC | ATCACACACGCATGGGCT | 59.40 | 59.65 | 216 | 63 |
100.00%
|
50.00%
|
| Primer 4429 | Gracilibacillus | TGTGATTGGTGGCAAGTGGA | AGCTTTCCCCATGTTCGGT | 59.82 | 59.23 | 218 | 63 |
100.00%
|
50.00%
|
| Primer 4430 | Gracilibacillus | TGCTCCACAATTTGCAGGC | TGCTTTAGGCCAACCGGT | 59.33 | 59.16 | 232 | 63 |
100.00%
|
50.00%
|