| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4451 | Gracilibacillus | GATTCCGCTCGATCCCGAA | ACCTTTTGGCAGCTGGCT | 59.64 | 59.80 | 247 | 63 |
100.00%
|
50.00%
|
| Primer 4452 | Gracilibacillus | ATCCCCTTCACCATCCCAC | TCCGGTGTCCATTTCTTGGT | 58.68 | 59.23 | 266 | 63 |
100.00%
|
50.00%
|
| Primer 4453 | Gracilibacillus | ATGCCGAAGCGCATCAGT | TCGGCTTCACGCTTGGTT | 60.12 | 59.89 | 160 | 63 |
100.00%
|
50.00%
|
| Primer 4454 | Gracilibacillus | TGCAGAGGGAGCCTAAGGT | CCATTTCGGGATGCTGCCT | 59.92 | 60.45 | 198 | 63 |
100.00%
|
50.00%
|
| Primer 4455 | Gracilibacillus | AACAGGCGACGACACGAA | ACCCCTCCTCTTTCCGACA | 59.59 | 59.84 | 276 | 63 |
100.00%
|
50.00%
|
| Primer 4456 | Gracilibacillus | TGGAATCACCAAGCGAAGGT | TCAGAATGATCCGCTGCCC | 59.60 | 59.85 | 296 | 63 |
100.00%
|
50.00%
|
| Primer 4457 | Gracilibacillus | TCATCGGAATGCTCGGGT | ATGCCAGCGGTTTTCGGA | 58.37 | 59.97 | 259 | 63 |
100.00%
|
50.00%
|
| Primer 4458 | Gracilibacillus | ATCATCGGAATGCTCGGGT | ATGCCAGCGGTTTTCGGA | 58.86 | 59.97 | 260 | 63 |
100.00%
|
50.00%
|
| Primer 4459 | Gracilibacillus | TGCCTGTCGTGATAATGGCT | ACCTACACCTGCATTCGGC | 59.46 | 60.08 | 236 | 63 |
100.00%
|
50.00%
|
| Primer 4460 | Gracilibacillus | ATTGGAGCGGACACAGTGG | TCTTCTGCCAGCGCTGTT | 60.00 | 59.57 | 215 | 63 |
100.00%
|
50.00%
|