| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4501 | Gracilibacillus | AACAGGCCATCGAGAAGCA | TGTTTGGACGCCTCAGCT | 59.32 | 59.17 | 195 | 63 |
100.00%
|
50.00%
|
| Primer 4502 | Gracilibacillus | AGGCCATCGAGAAGCAGT | TGTTTGGACGCCTCAGCT | 58.28 | 59.17 | 192 | 63 |
100.00%
|
50.00%
|
| Primer 4503 | Gracilibacillus | TTGTGCTGCCAACCAGGT | TAGGGTTGCTGGATTGCGG | 59.72 | 60.08 | 260 | 63 |
100.00%
|
50.00%
|
| Primer 4504 | Gracilibacillus | GGTGATCGTCTCCAAGAAGCT | TTTCTCGTCGGCTGCGTT | 59.79 | 59.97 | 156 | 63 |
100.00%
|
50.00%
|
| Primer 4505 | Gracilibacillus | AGGTGATCGTCTCCAAGAAGC | TTTCTCGTCGGCTGCGTT | 59.79 | 59.97 | 157 | 63 |
100.00%
|
50.00%
|
| Primer 4506 | Gracilibacillus | ACAATCGACACCTGCTGCT | ACTGCCTTGCGCTGTACT | 59.63 | 59.26 | 225 | 63 |
100.00%
|
50.00%
|
| Primer 4507 | Gracilibacillus | ATGGGCATCGCAACCACA | TGCTTGCCTTGTCCGGAA | 59.96 | 59.49 | 259 | 63 |
100.00%
|
50.00%
|
| Primer 4508 | Gracilibacillus | ATGGGTGGGCATCAAGCT | AGTGGTCCCCATCCCTTGA | 58.90 | 59.84 | 195 | 63 |
100.00%
|
50.00%
|
| Primer 4509 | Gracilibacillus | ACGGAAAGACGTCAAGAGGT | AGGATCCGCTTCAGCACTG | 58.96 | 59.78 | 276 | 63 |
100.00%
|
50.00%
|
| Primer 4510 | Gracilibacillus | TGCTTACAAGGCTGCGGT | TGACTTCCCGTGGCAGTT | 59.57 | 58.76 | 170 | 63 |
100.00%
|
50.00%
|