| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Gracilibacillus | TGCGCAGACAAAGTCGGA | ACGGACTCAGGCGATTGT | 59.58 | 58.62 | 156 | 140 |
100.00%
|
50.00%
|
| Primer 42 | Gracilibacillus | TGCGCAGACAAAGTCGGA | ACAACGGACTCAGGCGAT | 59.58 | 58.62 | 159 | 140 |
100.00%
|
50.00%
|
| Primer 43 | Gracilibacillus | TGCGCAGACAAAGTCGGA | AGTTGCCGTCTGAGGTGT | 59.58 | 58.45 | 251 | 140 |
100.00%
|
50.00%
|
| Primer 44 | Gracilibacillus | AATCCTGCCGTTCACGCA | TGTGAGGACCTGGTTTGCC | 59.97 | 59.85 | 208 | 138 |
100.00%
|
50.00%
|
| Primer 45 | Gracilibacillus | AATCCTGCCGTTCACGCA | TGAGGACCTGGTTTGCCTC | 59.97 | 59.24 | 206 | 138 |
100.00%
|
50.00%
|
| Primer 46 | Gracilibacillus | TACGCGTATGGGCACTTGG | AGCATTGCCAACCCTGCA | 60.15 | 60.20 | 288 | 138 |
100.00%
|
50.00%
|
| Primer 47 | Gracilibacillus | TGGGCACTTGGTCGATGT | AGCATTGCCAACCCTGCA | 58.84 | 60.20 | 280 | 138 |
100.00%
|
50.00%
|
| Primer 48 | Gracilibacillus | GGCGATGATTGGCAAGCA | AGCATTGCCAACCCTGCA | 58.80 | 60.20 | 185 | 138 |
100.00%
|
50.00%
|
| Primer 49 | Gracilibacillus | TGGCGATGATTGGCAAGC | AGCATTGCCAACCCTGCA | 58.80 | 60.20 | 186 | 138 |
100.00%
|
50.00%
|
| Primer 50 | Gracilibacillus | GGCGATGATTGGCAAGCAC | AGCATTGCCAACCCTGCA | 60.23 | 60.20 | 185 | 138 |
100.00%
|
50.00%
|