| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 5141 | Gracilibacillus | TCGCCAAGGGGAATTACCG | GGATTGACTGGCTCGGCAA | 59.78 | 60.38 | 228 | 59 |
100.00%
|
50.00%
|
| Primer 5142 | Gracilibacillus | TTCCGCAGTGACCTCCTTG | GCCATGATATCCTCGTCCGAT | 59.63 | 59.52 | 212 | 59 |
100.00%
|
50.00%
|
| Primer 5143 | Gracilibacillus | GTGGACGAAACGTAGCCCA | TCCGCACCAACAGGAACT | 60.01 | 58.76 | 214 | 59 |
100.00%
|
100.00%
|
| Primer 5144 | Gracilibacillus | ACCAAGGAAACACTGGCCA | TCCACCGCAACAATCGCA | 59.38 | 60.28 | 296 | 59 |
100.00%
|
50.00%
|
| Primer 5145 | Gracilibacillus | ACGTGATTGCTGCCACCA | AGCAACGACTTCGCGTGT | 59.89 | 60.28 | 194 | 59 |
100.00%
|
50.00%
|
| Primer 5146 | Gracilibacillus | GCGTAGAATCGTTGCTGCG | TACCGCTTGCCCAGGATT | 60.01 | 58.59 | 200 | 59 |
100.00%
|
50.00%
|
| Primer 5147 | Gracilibacillus | GCGTAGAATCGTTGCTGCG | ATACCGCTTGCCCAGGATT | 60.01 | 59.08 | 201 | 59 |
100.00%
|
50.00%
|
| Primer 5148 | Gracilibacillus | TATGAGCACCCCAATGGCC | TCCGACAATGCCTCCACAA | 59.77 | 59.24 | 212 | 59 |
100.00%
|
50.00%
|
| Primer 5149 | Gracilibacillus | TGGACAGCAGGCATTCCA | TGCGCTCGAATCCTAGCA | 58.83 | 58.79 | 249 | 59 |
100.00%
|
50.00%
|
| Primer 5150 | Gracilibacillus | CCTCAGGCAGCCTTCAAGT | ACGATGGGCTTGACGCAA | 59.62 | 59.97 | 221 | 59 |
100.00%
|
50.00%
|