| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 5271 | Gracilibacillus | GGCGTGCTTAATAATGGCAGG | CTGCTAGCATGCTAGAAGCTT | 60.00 | 58.16 | 185 | 58 |
100.00%
|
50.00%
|
| Primer 5272 | Gracilibacillus | GCGTGCGCTTCAAGTAGA | ATCGCTGGTCGGATCACA | 58.14 | 58.38 | 255 | 58 |
100.00%
|
50.00%
|
| Primer 5273 | Gracilibacillus | TCCGACAACCAAATAGCCAGT | ACTTCTACCCCAGCAACGC | 59.65 | 60.00 | 185 | 58 |
100.00%
|
50.00%
|
| Primer 5274 | Gracilibacillus | ACGTGGGATTTTACGAGGAGA | TTCAACGTCATCGGCTGGT | 58.83 | 59.63 | 228 | 58 |
100.00%
|
50.00%
|
| Primer 5275 | Gracilibacillus | ACGTGGGATTTTACGAGGAGA | GTCATCGGCTGGTTTGCA | 58.83 | 58.33 | 222 | 58 |
100.00%
|
50.00%
|
| Primer 5276 | Gracilibacillus | GCTGACGAACTTGATGTGCC | ACCGTTGCATCCCCTCTT | 59.83 | 58.50 | 193 | 58 |
100.00%
|
50.00%
|
| Primer 5277 | Gracilibacillus | ACTTTTGATGCAGCACACCA | TGCCCTTGAAGTGCTGCTT | 58.60 | 60.15 | 272 | 58 |
100.00%
|
50.00%
|
| Primer 5278 | Gracilibacillus | TGCAGCACACCATTTACACTG | TGCCCTTGAAGTGCTGCTT | 59.39 | 60.15 | 264 | 58 |
100.00%
|
50.00%
|
| Primer 5279 | Gracilibacillus | GCAGCACACCATTTACACTGT | TGCCCTTGAAGTGCTGCTT | 59.39 | 60.15 | 263 | 58 |
100.00%
|
50.00%
|
| Primer 5280 | Gracilibacillus | TGATGCTTTATCAGGTCCGC | AACCGTGCATGACTAGGTGA | 58.05 | 59.03 | 273 | 58 |
100.00%
|
50.00%
|