| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 561 | Gracilibacillus | CGAAGTGTGGGACGTGTCA | AAAGCCTACCGTAACGCGA | 59.93 | 59.41 | 299 | 109 |
100.00%
|
50.00%
|
| Primer 562 | Gracilibacillus | ACGTTGCAGATGAAGGCGA | GCGAAAGGCGTAAACGGT | 60.01 | 58.75 | 258 | 109 |
100.00%
|
50.00%
|
| Primer 563 | Gracilibacillus | AATCCCACAGCGGAACAGC | GCGTTTGTAGTCTCCAGCTG | 60.68 | 58.93 | 158 | 109 |
100.00%
|
50.00%
|
| Primer 564 | Gracilibacillus | ACAGGGCTTGGCTTTATGCT | AAGCCTATTGCGCTTCCGA | 59.96 | 59.78 | 227 | 109 |
100.00%
|
50.00%
|
| Primer 565 | Gracilibacillus | ACCAGGTATTTTCGCCTTACC | TGTCACCTCCATGATCGCTTT | 58.00 | 59.72 | 211 | 109 |
100.00%
|
50.00%
|
| Primer 566 | Gracilibacillus | ACTGCCAGCAATTGTCCGA | TCTCCGTAAACTCTGCGCA | 59.93 | 59.04 | 161 | 109 |
100.00%
|
50.00%
|
| Primer 567 | Gracilibacillus | TCACATGCGTGTCGAAGC | TGGCGTTGCTCCAATTTCC | 58.45 | 59.04 | 207 | 109 |
100.00%
|
50.00%
|
| Primer 568 | Gracilibacillus | ACCGCTTGCGTTCACGTA | TGCCTCAGCTGCTCGTTT | 59.97 | 59.57 | 266 | 109 |
100.00%
|
50.00%
|
| Primer 569 | Gracilibacillus | TGGCTGATCCAAACGCGA | AGCACGTTTACGCGTTGT | 59.65 | 58.29 | 190 | 109 |
100.00%
|
50.00%
|
| Primer 570 | Gracilibacillus | TCGACAGGCAGGTAACCGA | GCACCGCATGTTGCACAA | 60.60 | 59.97 | 215 | 109 |
100.00%
|
50.00%
|