| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 8001 | Gracilibacillus | ATTGGACGGCAGGGCTTT | CGTACCGCTTGGACGCATA | 59.56 | 60.23 | 218 | 43 |
100.00%
|
50.00%
|
| Primer 8002 | Gracilibacillus | AATTGGACGGCAGGGCTT | CGTACCGCTTGGACGCATA | 59.56 | 60.23 | 219 | 43 |
100.00%
|
50.00%
|
| Primer 8003 | Gracilibacillus | ATCGTGCGACAAGCCGAA | ATCGCAAGCATCGCCAGA | 60.05 | 59.81 | 188 | 43 |
100.00%
|
50.00%
|
| Primer 8004 | Gracilibacillus | TCGCAAAGCCGTCAAACA | TCCCCTCAATCACCGTGAGA | 58.19 | 60.25 | 152 | 43 |
100.00%
|
50.00%
|
| Primer 8005 | Gracilibacillus | ACAAGGGGACGCAGCAAA | ACGTTGCGAACCAGTACCC | 59.81 | 60.30 | 270 | 43 |
100.00%
|
50.00%
|
| Primer 8006 | Gracilibacillus | AGGTCTCTGCAGAACACGC | ACCGCTGCATCGTTTCCA | 60.01 | 59.97 | 274 | 43 |
100.00%
|
50.00%
|
| Primer 8007 | Gracilibacillus | GTGGGATGGGTGCCTTGAT | TCATGCGGAAGGTCCACTT | 59.69 | 58.93 | 161 | 43 |
100.00%
|
50.00%
|
| Primer 8008 | Gracilibacillus | TCATTCGGTGGGATGGGTG | TCATGCGGAAGGTCCACTT | 59.39 | 58.93 | 168 | 43 |
100.00%
|
50.00%
|
| Primer 8009 | Gracilibacillus | TGGGATGGGTGCCTTGAT | TCATGCGGAAGGTCCACTT | 58.15 | 58.93 | 160 | 43 |
100.00%
|
50.00%
|
| Primer 8010 | Gracilibacillus | AGGAACGAGCCTTGCAAGA | TTGCTTGGCCTCTTCGGT | 59.25 | 59.16 | 203 | 43 |
100.00%
|
50.00%
|