| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 8411 | Gracilibacillus | TCATGGCGACGCAAAACC | TCCCGTGTTGCAGCTGTT | 59.05 | 59.81 | 274 | 40 |
100.00%
|
50.00%
|
| Primer 8412 | Gracilibacillus | GCGCAATATGCAGCCGTT | ACACCATTGCTACAGCGGT | 59.59 | 59.63 | 286 | 40 |
100.00%
|
50.00%
|
| Primer 8413 | Gracilibacillus | TTTGTCGGTGCCTTGGCT | TACGAAACACCCCACAGCC | 59.81 | 59.93 | 289 | 40 |
100.00%
|
50.00%
|
| Primer 8414 | Gracilibacillus | TTTGTCGGTGCCTTGGCT | AAACACCCCACAGCCATCC | 59.81 | 60.23 | 285 | 40 |
100.00%
|
50.00%
|
| Primer 8415 | Gracilibacillus | TGTCAGACGGCAGAGCAAG | GCAACCCTCTCTGGCTGTT | 60.01 | 59.93 | 284 | 40 |
100.00%
|
50.00%
|
| Primer 8416 | Gracilibacillus | TGTCAGACGGCAGAGCAA | GCAACCCTCTCTGGCTGTT | 58.86 | 59.93 | 284 | 40 |
100.00%
|
50.00%
|
| Primer 8417 | Gracilibacillus | TTGTCAGACGGCAGAGCA | GCAACCCTCTCTGGCTGTT | 58.86 | 59.93 | 285 | 40 |
100.00%
|
50.00%
|
| Primer 8418 | Gracilibacillus | CACTGGGGTTACGGTGGTT | ACTGTGCAAGCCAGGGTT | 59.55 | 59.40 | 297 | 40 |
100.00%
|
50.00%
|
| Primer 8419 | Gracilibacillus | CACTGGGGTTACGGTGGTT | AACTGTGCAAGCCAGGGT | 59.55 | 59.40 | 298 | 40 |
100.00%
|
50.00%
|
| Primer 8420 | Gracilibacillus | AGCCAGCGCAGTCAAACT | TTGGCGAATTCCGTCCCT | 59.89 | 58.93 | 183 | 40 |
100.00%
|
50.00%
|