| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Halorhodospira | TGGTCAACCTGATCGCCAC | TGCTGGCCATGGTCATGT | 60.00 | 59.23 | 171 | 148 |
100.00%
|
100.00%
|
| Primer 2 | Halorhodospira | AAGACCCTGAACCCGATGC | ATATCCAGTGCGGTGGCTG | 59.70 | 59.85 | 248 | 146 |
100.00%
|
100.00%
|
| Primer 3 | Halorhodospira | AAGCTCCAGGTAACGCTGC | ACGCCTCCTGAACTTCCAG | 60.38 | 59.33 | 172 | 146 |
100.00%
|
100.00%
|
| Primer 4 | Halorhodospira | TGGTGGTGCTGATGCTCAG | ATACATCAGGGCAGCCGTG | 60.00 | 59.85 | 272 | 140 |
100.00%
|
100.00%
|
| Primer 5 | Halorhodospira | ACACCGCATCGATCAGCA | ATCGACGAACTGCGACTCC | 59.42 | 59.86 | 260 | 138 |
100.00%
|
100.00%
|
| Primer 6 | Halorhodospira | ACACCGCATCGATCAGCA | GGATCGACGAACTGCGACT | 59.42 | 59.86 | 262 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Halorhodospira | ACACCGCATCGATCAGCA | CGACGAACTGCGACTCCAT | 59.42 | 60.15 | 258 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Halorhodospira | TCTGCTGTTCGCGATGCA | ACCGATGCCACCCGTAATC | 60.05 | 59.85 | 211 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Halorhodospira | TCACAAGCACTGGATCGCC | GGCATCAGTATCGTCGGCA | 60.38 | 59.93 | 217 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Halorhodospira | GGCGAACGGTGAACAAACC | TTGCTCGACCCGTGGAAA | 60.01 | 59.18 | 279 | 137 |
100.00%
|
100.00%
|