| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1301 | Halothiobacillus | AGCTGAGGCGATCATGACG | ACCGCAACATGCAGACGT | 59.93 | 60.28 | 165 | 45 |
100.00%
|
100.00%
|
| Primer 1302 | Halothiobacillus | GCCTGGCACCTGGTTAACT | ACCGCAACATGCAGACGT | 59.93 | 60.28 | 251 | 45 |
100.00%
|
100.00%
|
| Primer 1303 | Halothiobacillus | AGGCCATTCGGTTGCACA | AAACGTGCCGTACCCACA | 59.88 | 59.49 | 202 | 45 |
100.00%
|
100.00%
|
| Primer 1304 | Halothiobacillus | TGCTGCTTGGCGTGATGA | GTGCAACGTCACGCCAAT | 59.97 | 59.36 | 153 | 45 |
100.00%
|
100.00%
|
| Primer 1305 | Halothiobacillus | TGACCATCATGACCTCGCC | TCGCTGATCGGCAAATCGT | 59.48 | 60.15 | 156 | 45 |
100.00%
|
100.00%
|
| Primer 1306 | Halothiobacillus | TGACCATCATGACCTCGCC | CGCTGATCGGCAAATCGT | 59.48 | 58.60 | 155 | 45 |
100.00%
|
100.00%
|
| Primer 1307 | Halothiobacillus | AGCCAGTTGGGTGCGATT | ACAAGCACACCAACCGCT | 59.56 | 60.12 | 286 | 45 |
100.00%
|
100.00%
|
| Primer 1308 | Halothiobacillus | AGCCAGTTGGGTGCGATT | AAGCACACCAACCGCTCA | 59.56 | 59.81 | 284 | 45 |
100.00%
|
100.00%
|
| Primer 1309 | Halothiobacillus | AGCCAGTTGGGTGCGATT | AGCACACCAACCGCTCAT | 59.56 | 59.57 | 283 | 45 |
100.00%
|
100.00%
|
| Primer 1310 | Halothiobacillus | TTCGGATTTGGCAGCGGT | TCGTGCACACCGAGCTTT | 59.97 | 59.89 | 203 | 45 |
100.00%
|
100.00%
|