| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 181 | Halothiobacillus | TCGACCGATTGACGCGAA | TCACGCTTGGCTGTTGCA | 59.44 | 60.52 | 157 | 86 |
100.00%
|
100.00%
|
| Primer 182 | Halothiobacillus | ATCGCTTTGCCTTCGGGT | GATTCGCCAGGTGCCAGAA | 59.65 | 60.38 | 163 | 86 |
100.00%
|
100.00%
|
| Primer 183 | Halothiobacillus | TACCTTGGTGCTAGCGCA | ACTCGCTCTTGCACCGAA | 58.62 | 59.27 | 224 | 86 |
100.00%
|
100.00%
|
| Primer 184 | Halothiobacillus | GCCCAACGCACTGCAAAT | TTGGTCGCAGGCGTTGAT | 59.66 | 59.97 | 196 | 86 |
100.00%
|
100.00%
|
| Primer 185 | Halothiobacillus | AACAGGCAAGGTCGTGCT | CACGACGGCACTAACGTTG | 59.49 | 59.51 | 213 | 86 |
100.00%
|
100.00%
|
| Primer 186 | Halothiobacillus | TGCGAAGAAGGCCATGCA | ACCTGTGCGCAACCCAAT | 59.97 | 60.20 | 159 | 86 |
100.00%
|
100.00%
|
| Primer 187 | Halothiobacillus | TTCGCTGCTGCTCATGCT | AGGGCTTGCAACCAAGGT | 60.05 | 59.39 | 185 | 86 |
100.00%
|
100.00%
|
| Primer 188 | Halothiobacillus | TTCGCTGCTGCTCATGCT | AAGGGCTTGCAACCAAGGT | 60.05 | 60.08 | 186 | 86 |
100.00%
|
100.00%
|
| Primer 189 | Halothiobacillus | TGCACAAGGGTTGCCAC | GAAAATCCGTGCGCACCA | 58.01 | 59.05 | 165 | 85 |
100.00%
|
100.00%
|
| Primer 190 | Halothiobacillus | TGCACAAGGGTTGCCAC | GAAAATCCGTGCGCACCA | 58.01 | 59.05 | 225 | 85 |
100.00%
|
100.00%
|