| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Halothiobacillus | CGAACGGCACAGAAAACCC | TGCCGTTGACGAGCACAT | 59.72 | 59.97 | 177 | 107 |
100.00%
|
100.00%
|
| Primer 32 | Halothiobacillus | AAGCAGCAAGGCAAGGCA | TTTGCGTTGCCGAACACG | 60.52 | 59.98 | 206 | 107 |
100.00%
|
100.00%
|
| Primer 33 | Halothiobacillus | AAAGCAGCAAGGCAAGGC | TTTGCGTTGCCGAACACG | 59.26 | 59.98 | 207 | 107 |
100.00%
|
100.00%
|
| Primer 34 | Halothiobacillus | GCAAGGCAAGGCAGGAAAC | TTTGCGTTGCCGAACACG | 60.01 | 59.98 | 201 | 107 |
100.00%
|
100.00%
|
| Primer 35 | Halothiobacillus | AAAAGCAGCAAGGCAAGGC | TTTGCGTTGCCGAACACG | 59.93 | 59.98 | 208 | 107 |
100.00%
|
100.00%
|
| Primer 36 | Halothiobacillus | AGGCAAGGCAGGAAACCTC | TTTGCGTTGCCGAACACG | 59.92 | 59.98 | 198 | 107 |
100.00%
|
100.00%
|
| Primer 37 | Halothiobacillus | AGCAAGGCAAGGCAGGAAA | TTTGCGTTGCCGAACACG | 60.15 | 59.98 | 202 | 107 |
100.00%
|
100.00%
|
| Primer 38 | Halothiobacillus | AGCAGCAAGGCAAGGCA | TTTGCGTTGCCGAACACG | 59.84 | 59.98 | 205 | 107 |
100.00%
|
100.00%
|
| Primer 39 | Halothiobacillus | TCTGCCATCACGCATGCA | GTGGCGATGAACAGGGTGA | 60.05 | 60.00 | 275 | 107 |
100.00%
|
100.00%
|
| Primer 40 | Halothiobacillus | ACGGGACCTTGCATCTTCC | AATCTCTGGACGGGAGGGA | 59.70 | 59.30 | 187 | 107 |
100.00%
|
100.00%
|