| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1231 | Holdemania | TACCTGCTGCAGCGCAAT | CCAGCCAATTCTGCAGGGA | 60.05 | 60.00 | 275 | 68 |
100.00%
|
33.33%
|
| Primer 1232 | Holdemania | TGTCATCGCCAGCTGGATC | TTGGCGATTTCCTGCGGT | 59.86 | 59.97 | 156 | 68 |
100.00%
|
33.33%
|
| Primer 1233 | Holdemania | GTGTCATCGCCAGCTGGAT | TTGGCGATTTCCTGCGGT | 60.15 | 59.97 | 157 | 68 |
100.00%
|
33.33%
|
| Primer 1234 | Holdemania | TGGATCGGTCCTGCTGGAT | TTTCTGCCCATCACCGCT | 60.38 | 59.24 | 186 | 68 |
100.00%
|
33.33%
|
| Primer 1235 | Holdemania | GCGCTGGCCAACTTACTAA | TGGCTGACCATCGATCACG | 58.15 | 59.86 | 174 | 67 |
100.00%
|
33.33%
|
| Primer 1236 | Holdemania | TGAACAGGGTGAGGTGCTG | ACCGACAACCACCGCAAA | 59.55 | 60.12 | 226 | 67 |
100.00%
|
33.33%
|
| Primer 1237 | Holdemania | CGCAGCAATGTCGGCAAA | TGGACGCATTGGCAAAGC | 59.75 | 59.35 | 166 | 67 |
100.00%
|
33.33%
|
| Primer 1238 | Holdemania | CGCAGCAATGTCGGCAAA | TTGGACGCATTGGCAAAGC | 59.75 | 60.01 | 167 | 67 |
100.00%
|
33.33%
|
| Primer 1239 | Holdemania | AGCCGTTCGCAGCTGAAA | GCAGGTCAGAGCTTCAGCA | 60.28 | 60.00 | 192 | 67 |
100.00%
|
33.33%
|
| Primer 1240 | Holdemania | AGCCGTTCGCAGCTGAAA | TGCAGGTCAGAGCTTCAGC | 60.28 | 60.00 | 193 | 67 |
100.00%
|
33.33%
|