| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1531 | Holdemania | TCCGCATCGCACGTTCTT | GGCCATGCGAAAACGGTT | 60.05 | 59.35 | 202 | 62 |
100.00%
|
33.33%
|
| Primer 1532 | Holdemania | TTCCGCATCGCACGTTCT | GGCCATGCGAAAACGGTT | 60.05 | 59.35 | 203 | 62 |
100.00%
|
33.33%
|
| Primer 1533 | Holdemania | GCTGCTGGGGTTATGCTGA | TTGGTCGTGCCGTGGAAA | 60.08 | 59.81 | 271 | 62 |
100.00%
|
33.33%
|
| Primer 1534 | Holdemania | TTGTCCGCTGTGCCGATT | GGATGGCTGAGGTTTCGCT | 59.97 | 60.08 | 165 | 62 |
100.00%
|
33.33%
|
| Primer 1535 | Holdemania | TTGTCCGCTGTGCCGATT | GCTGAGGTTTCGCTTTGGC | 59.97 | 60.08 | 160 | 62 |
100.00%
|
33.33%
|
| Primer 1536 | Holdemania | TTGTCCGCTGTGCCGATT | TGCCTGCCTGAAACTGGA | 59.97 | 58.75 | 211 | 62 |
100.00%
|
33.33%
|
| Primer 1537 | Holdemania | ACATCGTCGCTCTGTGCT | TGTTCATCGGCAGCGGTT | 59.03 | 59.97 | 210 | 62 |
100.00%
|
33.33%
|
| Primer 1538 | Holdemania | GGCAAACGGGAGATCGTGA | TCGCGTGCCGTTCATCAT | 60.08 | 60.13 | 279 | 62 |
100.00%
|
33.33%
|
| Primer 1539 | Holdemania | TGCTTTTGCCTGCCCAGT | ACTTTCGGTTTCGCCACCT | 60.12 | 59.85 | 217 | 62 |
100.00%
|
33.33%
|
| Primer 1540 | Holdemania | TGGTGTTGCGGATTGCCT | TCCAGTTGGTGCAGCTGT | 59.88 | 59.08 | 290 | 62 |
100.00%
|
33.33%
|