| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3251 | Holdemania | AGGCTGCTCTGGCGTTAA | AACAGCGCTGCGGATTCT | 58.93 | 60.05 | 237 | 27 |
100.00%
|
33.33%
|
| Primer 3252 | Holdemania | TGGCGTCCCAGCAATTCT | TGCCGAAAGCTGAGCGTA | 59.24 | 59.35 | 152 | 27 |
100.00%
|
33.33%
|
| Primer 3253 | Holdemania | GGCGTGGTGATTCAATCGC | TGACTGTCGCCAACGTGT | 59.94 | 59.50 | 286 | 25 |
100.00%
|
33.33%
|
| Primer 3254 | Holdemania | GGTGAACGAGAGCCTGCTT | AGCAGCGCATCGATCACA | 60.00 | 59.81 | 282 | 25 |
100.00%
|
33.33%
|
| Primer 3255 | Holdemania | ACACTCTGCCAGGATTCGC | TTATCGCCAGCCTGGAAGC | 60.08 | 60.15 | 279 | 25 |
100.00%
|
33.33%
|
| Primer 3256 | Holdemania | AGCGTGCTCAGATGCAGA | AGCCGTTCTGCGATCACA | 59.02 | 59.34 | 190 | 25 |
100.00%
|
33.33%
|
| Primer 3257 | Holdemania | TCGGTGCAATGGTCGCTT | AAGAACGCCAGCGCTACA | 59.97 | 59.66 | 217 | 25 |
100.00%
|
66.67%
|
| Primer 3258 | Holdemania | TGGAAAAGCTGCGGTCAGT | TGTCCTGACAGGTTTCCGC | 59.85 | 59.93 | 209 | 25 |
100.00%
|
33.33%
|
| Primer 3259 | Holdemania | TCAAGGCAAGGTGCTGCA | TGACAGCGCAATTCCCGA | 59.80 | 59.65 | 192 | 24 |
100.00%
|
33.33%
|
| Primer 3260 | Holdemania | CAGGAACAGCTGATCGACGA | AGCCCAGCGTTTCCACTT | 59.83 | 59.48 | 157 | 24 |
100.00%
|
66.67%
|