| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3281 | Holdemania | ACGCCACAACCAACACGA | TGAAGGTCAGGACACACGC | 60.13 | 59.93 | 189 | 24 |
100.00%
|
33.33%
|
| Primer 3282 | Holdemania | TTTGCGTATTGGGCAGCG | TCGGCACCGCTAAAAGCA | 59.13 | 59.97 | 225 | 24 |
100.00%
|
33.33%
|
| Primer 3283 | Holdemania | TTCTGCTGGATCCGGAAGC | TTCAACAGCCTGAGCCTCG | 59.78 | 60.00 | 281 | 24 |
100.00%
|
33.33%
|
| Primer 3284 | Holdemania | TCAGGCTTTACTGCTGGCC | ACGGCAAATTCAGCGTCG | 60.00 | 59.14 | 282 | 24 |
100.00%
|
33.33%
|
| Primer 3285 | Holdemania | ACTGGTTCGCTGGCGTTA | TGGCAATCTGCGTCAGCT | 59.26 | 59.65 | 157 | 23 |
100.00%
|
33.33%
|
| Primer 3286 | Holdemania | AGGCTTGTGCGTGCGTTA | AGCGGTTTGGCTGGGATT | 60.28 | 59.56 | 237 | 23 |
100.00%
|
33.33%
|
| Primer 3287 | Holdemania | TCGTTTGCGGACGCGTTA | AGCGCAAAACACAAGGCC | 60.35 | 59.58 | 218 | 23 |
100.00%
|
33.33%
|
| Primer 3288 | Holdemania | TGTTCTTAGCGGTGCTGGG | TCGGGAACAGGCAAAGCA | 60.00 | 59.49 | 288 | 23 |
100.00%
|
33.33%
|
| Primer 3289 | Holdemania | TGCTTCGCCACTGTGCAT | ACCTGGCTTCGCAGCTTA | 60.28 | 58.93 | 227 | 23 |
100.00%
|
33.33%
|
| Primer 3290 | Holdemania | AACGCGTCGCTGAGCTTA | TGCCCTCGCGTTCTTCAA | 59.74 | 59.58 | 265 | 23 |
100.00%
|
33.33%
|