| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Holdemania | TGTGCGTGCTGTCGTTGT | TTCCTGCCATTCGCCTGT | 60.51 | 59.24 | 239 | 117 |
100.00%
|
66.67%
|
| Primer 52 | Holdemania | CGTCAGGAAGCGGTTGTCT | TTCCCGTTGCAAGCGTCA | 60.01 | 60.20 | 256 | 117 |
100.00%
|
33.33%
|
| Primer 53 | Holdemania | TCGACGTTTCTGCGGTGT | CCAGCACGGTTAAATGCGG | 59.59 | 59.86 | 166 | 115 |
100.00%
|
33.33%
|
| Primer 54 | Holdemania | TTGGCAAGCGCTGTCTGT | CATCGCGATTCGTACCCCA | 60.20 | 59.93 | 158 | 115 |
100.00%
|
33.33%
|
| Primer 55 | Holdemania | TCCGTCAGGAAGTCGACCA | AGCGTTAGTTTTGGTGCGC | 60.23 | 59.72 | 152 | 115 |
100.00%
|
33.33%
|
| Primer 56 | Holdemania | TGCAGCGTGAACAGCTTC | CCCTTCAATGATCGCCTGC | 58.67 | 58.97 | 173 | 114 |
100.00%
|
66.67%
|
| Primer 57 | Holdemania | CTTTGTGCGTCAGGAAGCG | TTCCCGTTGCAAGCGTCA | 59.79 | 60.20 | 263 | 114 |
100.00%
|
33.33%
|
| Primer 58 | Holdemania | TGCAGCCAACGTGGAAGT | TCCTTTCTGATCCGCTGCG | 59.81 | 60.15 | 268 | 114 |
100.00%
|
33.33%
|
| Primer 59 | Holdemania | TCCGTCAGGAAGTCGACCA | TTCGCTACTGTACGACGCG | 60.23 | 60.23 | 187 | 114 |
100.00%
|
66.67%
|
| Primer 60 | Holdemania | AGCCTTGCACTTCGGTGT | TGTAGTCGCCATCTGCTCG | 59.49 | 59.57 | 154 | 114 |
100.00%
|
33.33%
|