| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Kluyvera | ATTTGCCAGCCCACGGTT | AATCCGCAGCCGAACGTT | 60.20 | 60.36 | 293 | 89 |
100.00%
|
50.00%
|
| Primer 42 | Kluyvera | TGCTGCATGGACGTTGGT | GCGGTGATTTTGCACGGTT | 59.89 | 60.01 | 152 | 89 |
100.00%
|
50.00%
|
| Primer 43 | Kluyvera | TGACGTCCGTTTTGCCCT | GCTGCTGCGGTTTGGTTT | 59.49 | 59.58 | 233 | 87 |
100.00%
|
50.00%
|
| Primer 44 | Kluyvera | ACTTTACGCCTGCGCAGT | CGTTCAGCTCGTTTGCCAC | 59.97 | 60.08 | 240 | 87 |
100.00%
|
50.00%
|
| Primer 45 | Kluyvera | ATTTGCCAGCCCACGGTT | TCCGCAGCCGAACGTTAT | 60.20 | 59.43 | 291 | 86 |
100.00%
|
50.00%
|
| Primer 46 | Kluyvera | TGCAAACCGCTGACCACA | TGGCGACGAACATGGCAA | 60.12 | 60.28 | 165 | 86 |
100.00%
|
100.00%
|
| Primer 47 | Kluyvera | TGCAAACCGCTGACCACA | ACGAACATGGCAACCGGT | 60.12 | 59.89 | 160 | 84 |
100.00%
|
100.00%
|
| Primer 48 | Kluyvera | AATGGAAGGCGTGCCGAA | CTGATGAATCGCGTGCAGC | 59.97 | 60.01 | 154 | 84 |
100.00%
|
50.00%
|
| Primer 49 | Kluyvera | TTCTGCGCATCACTGGCA | GCAGCAGCAGGGCAAAAT | 59.97 | 59.34 | 209 | 84 |
100.00%
|
100.00%
|
| Primer 50 | Kluyvera | TGCTGAAATTTCGCTGCGG | CGCGTTCAAAGGCTTGCT | 59.79 | 59.36 | 168 | 84 |
100.00%
|
50.00%
|