| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Lachnospira | CGCAATTGCAAGAGCGCT | ACCCTTTTCCTGATCGCTGA | 59.82 | 59.02 | 229 | 50 |
100.00%
|
100.00%
|
| Primer 522 | Lachnospira | CGCAATTGCAAGAGCGCT | CACCCTTTTCCTGATCGCTG | 59.82 | 58.91 | 230 | 50 |
100.00%
|
100.00%
|
| Primer 523 | Lachnospira | TCCGGAGTTTGGCAGACTG | TGAGTGGCTTTGCTGTGCT | 59.63 | 60.15 | 233 | 50 |
100.00%
|
100.00%
|
| Primer 524 | Lachnospira | AGGGCAATGGCAGCAGAT | GTCCGTCTTGGCAACCGTA | 59.31 | 60.01 | 201 | 50 |
100.00%
|
50.00%
|
| Primer 525 | Lachnospira | TGGAGCAGCGTGGAAAGAA | ACGGGTGCTGTGAGTGAT | 59.55 | 58.53 | 299 | 50 |
100.00%
|
50.00%
|
| Primer 526 | Lachnospira | AGGTTTCAGCAGCAGCCA | GGAACTCTGCGCTCTGGAA | 59.48 | 59.71 | 216 | 50 |
100.00%
|
50.00%
|
| Primer 527 | Lachnospira | AGGTGCGGTTCGTGAGTT | GCGTCGCTGTTCTGATGGA | 59.18 | 60.45 | 244 | 50 |
100.00%
|
50.00%
|
| Primer 528 | Lachnospira | GTGGGCATGTTCAGGTGGT | GCAGACGCACCAGACTGAA | 60.23 | 60.30 | 285 | 50 |
100.00%
|
50.00%
|
| Primer 529 | Lachnospira | GTGGGCATGTTCAGGTGGT | CGCACCAGACTGAAATGCC | 60.23 | 59.50 | 280 | 50 |
100.00%
|
50.00%
|
| Primer 530 | Lachnospira | GTGGCATATACCGCTGGCT | TCTGGAACAACCACGGCAA | 59.93 | 59.78 | 235 | 50 |
100.00%
|
50.00%
|