| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lacticaseibacillus | AGAACAAGGCGATGTCCCC | GGGCTGAAATGGTTGGGTG | 59.70 | 59.03 | 151 | 140 |
100.00%
|
22.22%
|
| Primer 2 | Lacticaseibacillus | AGCATCCGGATTCCGTGTC | TGCGTTTCGTCCTTGGCA | 59.86 | 60.20 | 270 | 138 |
100.00%
|
33.33%
|
| Primer 3 | Lacticaseibacillus | GCAGGATTGGCCAAGTTGC | ACATCCGCTCGCATCACA | 60.08 | 59.42 | 164 | 137 |
100.00%
|
55.56%
|
| Primer 4 | Lacticaseibacillus | CGCCTCTTTTGTGCTCACC | ACCGTTCGGCATCCCAAA | 59.42 | 59.57 | 278 | 137 |
100.00%
|
55.56%
|
| Primer 5 | Lacticaseibacillus | CTTTTGTGCTCACCCGTGG | ACCGTTCGGCATCCCAAA | 59.35 | 59.57 | 273 | 137 |
100.00%
|
55.56%
|
| Primer 6 | Lacticaseibacillus | GCAGGATTGGCCAAGTTGC | ACGTGCCCTTCTTGGTTGG | 60.08 | 60.53 | 226 | 135 |
100.00%
|
55.56%
|
| Primer 7 | Lacticaseibacillus | TGTTGACCGAGTTTGGCCA | TGCGTTTCGTCCTTGGCA | 59.78 | 60.20 | 249 | 135 |
100.00%
|
33.33%
|
| Primer 8 | Lacticaseibacillus | TGCGGAAGTGGTTTGGTCA | TGCGTTTCGTCCTTGGCA | 59.78 | 60.20 | 163 | 132 |
100.00%
|
33.33%
|
| Primer 9 | Lacticaseibacillus | TGGCAATGGGCATGTTGC | TCCACAAAGGCCACGTCA | 59.34 | 59.09 | 280 | 132 |
100.00%
|
22.22%
|
| Primer 10 | Lacticaseibacillus | TGGATGGCTGCCTTTTCGG | ACCGTTCGGCATCCCAAA | 60.68 | 59.57 | 252 | 130 |
100.00%
|
55.56%
|