| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lactococcus | GGAGCGCTTTTGACTGATGC | TTCTTCGGCAGCTTCATCAG | 60.18 | 58.27 | 246 | 157 |
100.00%
|
30.00%
|
| Primer 2 | Lactococcus | AGCGCTTTTGACTGATGCAG | TTCTTCGGCAGCTTCATCAG | 59.48 | 58.27 | 244 | 148 |
100.00%
|
30.00%
|
| Primer 3 | Lactococcus | CGCAACTAAAGGCTTCGGC | ACAGCTTGAGGTCCACGAG | 59.87 | 59.33 | 153 | 143 |
100.00%
|
50.00%
|
| Primer 4 | Lactococcus | CGCAACTAAAGGCTTCGGC | AACAGCTTGAGGTCCACGAG | 59.87 | 59.97 | 154 | 143 |
100.00%
|
50.00%
|
| Primer 5 | Lactococcus | TGGGCAGAAGATGAAGCAGG | AGGGCTGTGCATGATGAGC | 60.03 | 60.45 | 231 | 143 |
100.00%
|
30.00%
|
| Primer 6 | Lactococcus | CGCAACTAAAGGCTTCGGC | ACAGCTTGAGGTCCACGAG | 59.87 | 59.33 | 153 | 143 |
100.00%
|
50.00%
|
| Primer 7 | Lactococcus | CGCAACTAAAGGCTTCGGC | AACAGCTTGAGGTCCACGAG | 59.87 | 59.97 | 154 | 143 |
100.00%
|
50.00%
|
| Primer 8 | Lactococcus | TGTTGTTGAAGCACCTGCA | CGTAAACGCGAAGACCTGG | 58.12 | 58.93 | 197 | 138 |
100.00%
|
50.00%
|
| Primer 9 | Lactococcus | TGTCCATGCTGCTTGGCA | GCGCTTGAATCACACCGTC | 59.88 | 59.87 | 170 | 138 |
100.00%
|
30.00%
|
| Primer 10 | Lactococcus | TGGGAGGACACAAAGCAGG | ACATGGGCAGAAACGAGCA | 59.54 | 59.93 | 244 | 138 |
100.00%
|
30.00%
|