| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Lactococcus | TGATGGCATGGGAGGACAC | ACATGGGCAGAAACGAGCA | 59.39 | 59.93 | 252 | 138 |
100.00%
|
30.00%
|
| Primer 12 | Lactococcus | AGCTGATGGCATGGGAGGA | ACATGGGCAGAAACGAGCA | 60.70 | 59.93 | 255 | 138 |
100.00%
|
30.00%
|
| Primer 13 | Lactococcus | TGGCCTGGACGCATTTGT | AATGCGGTGTGGACCACTC | 59.88 | 60.30 | 157 | 137 |
100.00%
|
30.00%
|
| Primer 14 | Lactococcus | GATGGCATGGGAGGACACA | ACATGGGCAGAAACGAGCA | 59.39 | 59.93 | 251 | 137 |
100.00%
|
30.00%
|
| Primer 15 | Lactococcus | TGCAGCATTGGACCACGAT | TCACGGCTCTATCCCACCA | 60.00 | 60.00 | 163 | 135 |
100.00%
|
30.00%
|
| Primer 16 | Lactococcus | TGTCCATGCTGCTTGGCA | AGCGCTTGAATCACACCG | 59.88 | 58.43 | 171 | 135 |
100.00%
|
30.00%
|
| Primer 17 | Lactococcus | TGTCCATGCTGCTTGGCA | AAGCGCTTGAATCACACCG | 59.88 | 59.13 | 172 | 135 |
100.00%
|
30.00%
|
| Primer 18 | Lactococcus | TGCAAATGGTTTCCGCTGC | ACCGACAAGTCCACGATCC | 60.01 | 59.41 | 200 | 135 |
100.00%
|
30.00%
|
| Primer 19 | Lactococcus | TGCAAATGGTTTCCGCTGC | TAGCCACCGACAAGTCCAC | 60.01 | 59.33 | 205 | 135 |
100.00%
|
30.00%
|
| Primer 20 | Lactococcus | TGCAGCAAACCAACTTGGC | AGCATGGCGAACGACGTA | 59.86 | 59.43 | 232 | 133 |
100.00%
|
40.00%
|