| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Lancefieldella | TCGTCAGGCGTTTGCTCA | ACCGCACGCGAGAAAATCA | 59.58 | 60.67 | 297 | 132 |
100.00%
|
50.00%
|
| Primer 22 | Lancefieldella | CGGTGTTGCTTGTTCTGGC | GCGCAAACCAGGAAGATGG | 60.01 | 59.49 | 154 | 132 |
100.00%
|
50.00%
|
| Primer 23 | Lancefieldella | ACGCAAACTCAGCTGGCA | ACGCGTGGAAGCTTCTCA | 60.20 | 59.27 | 244 | 132 |
100.00%
|
50.00%
|
| Primer 24 | Lancefieldella | ACGCAAACTCAGCTGGCA | AACGCGTGGAAGCTTCTCA | 60.20 | 59.93 | 245 | 132 |
100.00%
|
50.00%
|
| Primer 25 | Lancefieldella | AGGCTGTAGCACGCACAA | TGCCAACGCGAACAACAC | 59.57 | 59.59 | 198 | 130 |
100.00%
|
50.00%
|
| Primer 26 | Lancefieldella | TCACGCAGAAACGGTGCA | TGCGCTCGATAACAGGCAA | 60.20 | 60.08 | 182 | 130 |
100.00%
|
25.00%
|
| Primer 27 | Lancefieldella | TCGTCAGGCGTTTGCTCA | CCGCACGCGAGAAAATCAA | 59.58 | 59.50 | 296 | 130 |
100.00%
|
50.00%
|
| Primer 28 | Lancefieldella | ACGCAAACTCAGCTGGCA | GCGGTACCAAGACCACCAA | 60.20 | 59.93 | 265 | 130 |
100.00%
|
75.00%
|
| Primer 29 | Lancefieldella | CTGATGGAGCAGGGCAAGT | CCAGCAGCGAGGGTAACAA | 59.70 | 60.00 | 203 | 130 |
100.00%
|
50.00%
|
| Primer 30 | Lancefieldella | TGGAGCAGGGCAAGTACAC | CCAGCAGCGAGGGTAACAA | 59.63 | 60.00 | 199 | 130 |
100.00%
|
50.00%
|