| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Lancefieldella | AACCGCTCGTCGCATCAT | ACGCTCATACTGCTCTGCC | 59.82 | 59.86 | 181 | 127 |
100.00%
|
75.00%
|
| Primer 52 | Lancefieldella | ACCAAGCGGTGTTGCAAC | CTGAAGCTCACGGAAGGCA | 59.20 | 60.00 | 247 | 127 |
100.00%
|
50.00%
|
| Primer 53 | Lancefieldella | ACCAAGCGGTGTTGCAAC | TGAAGCTCACGGAAGGCA | 59.20 | 58.85 | 246 | 127 |
100.00%
|
50.00%
|
| Primer 54 | Lancefieldella | ACCAAGCGGTGTTGCAAC | TTCCCTCAGCCATGTGCA | 59.20 | 58.83 | 182 | 127 |
100.00%
|
50.00%
|
| Primer 55 | Lancefieldella | TGGTCGCATGTGCAAACG | TGCAGCACCGGTTTCCTT | 59.36 | 59.81 | 275 | 125 |
100.00%
|
50.00%
|
| Primer 56 | Lancefieldella | TGGCACGTCAAGTTGGCA | ACGTATCCCAAGGAAGCGC | 60.12 | 60.15 | 256 | 125 |
100.00%
|
25.00%
|
| Primer 57 | Lancefieldella | GGTGGAGCAAAGAAGGGCT | ACCACAGCGTATCACTGCTT | 59.92 | 59.68 | 226 | 125 |
100.00%
|
50.00%
|
| Primer 58 | Lancefieldella | AAACGCGTGATGCTCAAGC | TGCGAACCGTGCTTTCCA | 59.79 | 60.20 | 219 | 124 |
100.00%
|
50.00%
|
| Primer 59 | Lancefieldella | GGTGGAGCAAAGAAGGGCT | CTCTGCAATTGACCACAGCG | 59.92 | 59.83 | 237 | 124 |
100.00%
|
50.00%
|
| Primer 60 | Lancefieldella | GGTGGAGCAAAGAAGGGCT | TCTGCAATTGACCACAGCG | 59.92 | 58.75 | 236 | 124 |
100.00%
|
50.00%
|