| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Lawsonia | GCAGCATCGACAAAGTGGC | GTCCCAGACTTCAAGGCCAA | 60.15 | 59.89 | 191 | 130 |
100.00%
|
100.00%
|
| Primer 32 | Lawsonia | ACCATCCTGAGCTTGTCCTTG | CGGGTGCTTTTGGATTCCC | 60.00 | 59.11 | 183 | 129 |
100.00%
|
100.00%
|
| Primer 33 | Lawsonia | GCTACCTTGCCCAACAGGA | ACCCATAACTGCTGGCTCTG | 59.62 | 59.75 | 253 | 129 |
100.00%
|
100.00%
|
| Primer 34 | Lawsonia | TTCACACGTTGGCCTGACC | CGTGCTACTCCTGGAAGAGTG | 60.53 | 60.14 | 194 | 129 |
100.00%
|
100.00%
|
| Primer 35 | Lawsonia | TGAGGTTATGGAAGGTGGGGA | ACACCTGTTGCTACTGCTGT | 60.20 | 59.53 | 282 | 129 |
100.00%
|
100.00%
|
| Primer 36 | Lawsonia | CGCACCAAACTCCGCAAT | ACACGGCATTGTTGTCCATC | 59.05 | 59.12 | 227 | 128 |
100.00%
|
100.00%
|
| Primer 37 | Lawsonia | CGCACCAAACTCCGCAAT | AGTACACGGCATTGTTGTCCA | 59.05 | 60.20 | 230 | 128 |
100.00%
|
100.00%
|
| Primer 38 | Lawsonia | CGCACCAAACTCCGCAAT | GTACACGGCATTGTTGTCCA | 59.05 | 58.77 | 229 | 128 |
100.00%
|
100.00%
|
| Primer 39 | Lawsonia | CGCACCAAACTCCGCAAT | AGTACACGGCATTGTTGTCC | 59.05 | 58.48 | 230 | 128 |
100.00%
|
100.00%
|
| Primer 40 | Lawsonia | CGCACCAAACTCCGCAAT | GTACACGGCATTGTTGTCCAT | 59.05 | 59.19 | 229 | 128 |
100.00%
|
100.00%
|