| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 491 | Legionella | AGCCATGCGCATTTTGCC | AAGACAGCCGTTCCCTTGG | 60.13 | 59.93 | 163 | 85 |
100.00%
|
80.00%
|
| Primer 492 | Legionella | ACTGCGTAAATGGTTGGCC | AAGCCAACCATCACCCCAG | 58.74 | 59.92 | 210 | 85 |
100.00%
|
40.00%
|
| Primer 493 | Legionella | AGAGCTCATCTGAAGACGAGA | GCAGTAATCACCAAAGTGGCC | 58.00 | 59.80 | 217 | 85 |
100.00%
|
40.00%
|
| Primer 494 | Legionella | CTCGCTGTTTTGGACGTTGA | ACGAGAAAGCAGCGCAGT | 59.07 | 59.97 | 237 | 85 |
100.00%
|
40.00%
|
| Primer 495 | Legionella | TGAAACGCGAACAAGTGATTG | TCTTGCCATACCTCCTGCTT | 58.02 | 58.71 | 152 | 85 |
100.00%
|
40.00%
|
| Primer 496 | Legionella | TCCCAAAACGGACTGTGTCA | TGCCGTTGTTGTGGAGCA | 59.46 | 60.12 | 296 | 85 |
100.00%
|
40.00%
|
| Primer 497 | Legionella | GGAAATGGCTGTGTTCCGC | ATTGGTGGGCAAGACGCA | 59.79 | 59.88 | 171 | 85 |
100.00%
|
40.00%
|
| Primer 498 | Legionella | GGCTGTGTTCCGCAAAAGA | ATTGGTGGGCAAGACGCA | 58.97 | 59.88 | 165 | 85 |
100.00%
|
40.00%
|
| Primer 499 | Legionella | AGCCGTGGGTGTTTCTGA | ACCCAACTGTCTTGGCCA | 58.76 | 58.66 | 219 | 85 |
100.00%
|
40.00%
|
| Primer 500 | Legionella | TTTGCAAGGCGAGTGGGA | ATCCGTGCGACAGTTGCA | 59.49 | 59.97 | 191 | 85 |
100.00%
|
40.00%
|