| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1221 | Leptospira | GTTTCCCTTCGAGGACCCA | ACGTCCACTGAGCGCAAT | 58.94 | 59.66 | 270 | 84 |
100.00%
|
50.00%
|
| Primer 1222 | Leptospira | TGTTTCCCTTCGAGGACCC | ACGTCCACTGAGCGCAAT | 58.94 | 59.66 | 271 | 84 |
100.00%
|
50.00%
|
| Primer 1223 | Leptospira | GGTCCATCTTGGCAACGGA | GCCATAAGCAGCTCCCGAA | 60.00 | 60.15 | 287 | 84 |
100.00%
|
50.00%
|
| Primer 1224 | Leptospira | GGTCCATCTTGGCAACGGA | AAGCAGCTCCCGAACTTGT | 60.00 | 59.55 | 282 | 84 |
100.00%
|
50.00%
|
| Primer 1225 | Leptospira | AAACGCGAGAAGTCCGGA | GCCCATACGCACATAGGCT | 58.95 | 59.93 | 161 | 84 |
100.00%
|
50.00%
|
| Primer 1226 | Leptospira | AATGTCCGTGGTCGCGAT | TGTCAGGACACGGTCAGGA | 59.42 | 60.15 | 240 | 84 |
100.00%
|
50.00%
|
| Primer 1227 | Leptospira | CGTTCGGAAACACCACCTG | CACGCCAATCCAACGGATC | 59.06 | 59.28 | 204 | 84 |
100.00%
|
50.00%
|
| Primer 1228 | Leptospira | ATAGCTGCCATGTCCGCTC | ACGCCAACCTCCATCGAAG | 59.93 | 60.08 | 155 | 84 |
100.00%
|
50.00%
|
| Primer 1229 | Leptospira | TGTGGCAGTCGCTGATGT | TCAACGGGTGGAACGGAA | 59.26 | 58.77 | 291 | 84 |
100.00%
|
50.00%
|
| Primer 1230 | Leptospira | TGTGTGGCAGTCGCTGAT | TCAACGGGTGGAACGGAA | 59.26 | 58.77 | 293 | 84 |
100.00%
|
50.00%
|