| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1921 | Leptospira | TGATCGCACAACCGCAAA | AAGGCGACTGAAAACCAACAA | 58.26 | 58.90 | 253 | 74 |
100.00%
|
50.00%
|
| Primer 1922 | Leptospira | TGGACCAGGCAATTTGGGA | TCGCGTGGTCCTTTCCAA | 59.14 | 59.18 | 229 | 74 |
100.00%
|
50.00%
|
| Primer 1923 | Leptospira | TCGCCTATGCCCTGTTTGT | TCGGTTGGCCAAAGGGAT | 59.32 | 58.82 | 194 | 74 |
100.00%
|
50.00%
|
| Primer 1924 | Leptospira | AACCTGCCATTGTGATCGG | CGATCGCGTATGCATCTGC | 58.13 | 59.59 | 186 | 74 |
100.00%
|
50.00%
|
| Primer 1925 | Leptospira | TGGCAGCGTTTGGGTTTG | TTCACTTCGCCAGACCGAC | 59.19 | 60.01 | 193 | 74 |
100.00%
|
50.00%
|
| Primer 1926 | Leptospira | AGCCAACCTTTCCAAACTCC | ACTCCGCAATCCCTTTCGG | 58.30 | 60.08 | 197 | 74 |
100.00%
|
50.00%
|
| Primer 1927 | Leptospira | TGGGGTGAACGAGCCTTTC | ACCAATCGCACCACCCAA | 59.93 | 59.48 | 291 | 74 |
100.00%
|
50.00%
|
| Primer 1928 | Leptospira | CCGCATTCCTTGGTGGGAT | ACCAATCGCACCACCCAA | 60.08 | 59.48 | 254 | 74 |
100.00%
|
50.00%
|
| Primer 1929 | Leptospira | TGGGCAAGGTTACGATCGG | TGCACCCACAAACGACCA | 59.78 | 59.73 | 278 | 74 |
100.00%
|
50.00%
|
| Primer 1930 | Leptospira | TCCCAGGGACTTCTGCCAA | AGCCCAGCGACAATTGCA | 60.46 | 60.28 | 298 | 74 |
100.00%
|
50.00%
|