| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3331 | Leptospira | GGAATGAGATCGTCGGAGAGG | ATGGGTATGCGATGGCGT | 59.73 | 59.17 | 159 | 56 |
100.00%
|
50.00%
|
| Primer 3332 | Leptospira | AGCTCCAAAAGGTGCCGT | AGGAGCGCTTGCATTCCA | 59.48 | 59.65 | 198 | 56 |
100.00%
|
50.00%
|
| Primer 3333 | Leptospira | TGCCAACGCCAGCCTATT | TGGTACGGCTGGTTGCAAA | 59.65 | 60.15 | 191 | 56 |
100.00%
|
50.00%
|
| Primer 3334 | Leptospira | TGCCAACGCCAGCCTATT | TTGGTACGGCTGGTTGCAA | 59.65 | 60.15 | 192 | 56 |
100.00%
|
50.00%
|
| Primer 3335 | Leptospira | TGGCCTGGCGTTTTGGTT | AGTGCGGAGACTGGCATT | 60.44 | 58.93 | 170 | 56 |
100.00%
|
50.00%
|
| Primer 3336 | Leptospira | TTGGCCTGGCGTTTTGGT | AGTGCGGAGACTGGCATT | 60.44 | 58.93 | 171 | 56 |
100.00%
|
50.00%
|
| Primer 3337 | Leptospira | TGGCCTGGCGTTTTGGTT | GAGTGCGGAGACTGGCATT | 60.44 | 60.08 | 171 | 56 |
100.00%
|
50.00%
|
| Primer 3338 | Leptospira | TTGGCCTGGCGTTTTGGT | GAGTGCGGAGACTGGCATT | 60.44 | 60.08 | 172 | 56 |
100.00%
|
50.00%
|
| Primer 3339 | Leptospira | CACTGGTGGGAATGCTCGT | AAGGGCACTCGATTGGCA | 60.00 | 59.24 | 243 | 56 |
100.00%
|
50.00%
|
| Primer 3340 | Leptospira | ATCTCTGCCGATGTTCGCC | TGGTCCACAACACCACGT | 60.23 | 59.01 | 170 | 56 |
100.00%
|
50.00%
|