| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4261 | Leptospira | GCGATTGGCCTTGTTCACG | TGATCACCCCATCCGGAGT | 60.15 | 60.00 | 208 | 44 |
100.00%
|
50.00%
|
| Primer 4262 | Leptospira | GGATTCTTGCACCTGCGAC | GGAGGAAGCGCTTCTCCTT | 59.20 | 59.40 | 211 | 44 |
100.00%
|
50.00%
|
| Primer 4263 | Leptospira | TGCGGAACTGCTCGGAAA | CCTTGTTCCCGATCGGCTT | 59.58 | 60.08 | 197 | 44 |
100.00%
|
50.00%
|
| Primer 4264 | Leptospira | CTTTGTTTCGCGACCGCA | TCGAAAGCCCATTCCGGAG | 59.37 | 59.78 | 194 | 44 |
100.00%
|
50.00%
|
| Primer 4265 | Leptospira | CGCTCTGCATTCTCCCCTT | TGGGCTCATCCGTCGAAA | 59.78 | 58.62 | 188 | 44 |
100.00%
|
50.00%
|
| Primer 4266 | Leptospira | CGCTCTGCATTCTCCCCTT | ATGGGCTCATCCGTCGAA | 59.78 | 58.37 | 189 | 44 |
100.00%
|
50.00%
|
| Primer 4267 | Leptospira | TCCGGTTCCGAATGCGAA | ACGCGATTGGAACGGAAGA | 59.34 | 59.71 | 154 | 44 |
100.00%
|
50.00%
|
| Primer 4268 | Leptospira | TCCGGTTCCGAATGCGAAA | ACGCGATTGGAACGGAAGA | 60.00 | 59.71 | 154 | 44 |
100.00%
|
50.00%
|
| Primer 4269 | Leptospira | TCGCAGGCGATGTGATCA | ACACTCGCTTGTGTCGCTT | 59.11 | 60.23 | 256 | 44 |
100.00%
|
50.00%
|
| Primer 4270 | Leptospira | ATCGGTTCCGGTCAGCTT | CCACGATTTCACGGAGGGA | 58.61 | 59.41 | 151 | 44 |
100.00%
|
50.00%
|