| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1251 | Massilioclostridium | TCTCGCCAGAACGCCAAA | CCATGCCGTGGTACAACCT | 59.58 | 60.00 | 238 | 49 |
100.00%
|
100.00%
|
| Primer 1252 | Massilioclostridium | TTCTCGCCAGAACGCCAA | CCATGCCGTGGTACAACCT | 59.58 | 60.00 | 239 | 49 |
100.00%
|
100.00%
|
| Primer 1253 | Massilioclostridium | AAAAGGGCTGGTGCAGGT | CCATGCCGTGGTACAACCT | 59.39 | 60.00 | 197 | 49 |
100.00%
|
100.00%
|
| Primer 1254 | Massilioclostridium | ATTCTCGCCAGAACGCCA | CCATGCCGTGGTACAACCT | 59.34 | 60.00 | 240 | 49 |
100.00%
|
100.00%
|
| Primer 1255 | Massilioclostridium | AACATTGGGCAGCTCACCA | AAGGGCAACAGTACGCAGG | 59.85 | 60.30 | 156 | 49 |
100.00%
|
100.00%
|
| Primer 1256 | Massilioclostridium | AACAGCGACAACCCAGAGG | TTTCCGCAGCAGCTTGGA | 59.93 | 59.89 | 291 | 49 |
100.00%
|
100.00%
|
| Primer 1257 | Massilioclostridium | ACAGATGCCGATTCCACCC | AGGCGATACCAGCACTTGC | 59.78 | 60.45 | 167 | 49 |
100.00%
|
100.00%
|
| Primer 1258 | Massilioclostridium | ACAGCCAGCAGACGCAAA | GGCATTGATACGCGTTTCCT | 60.20 | 58.99 | 272 | 49 |
100.00%
|
100.00%
|
| Primer 1259 | Massilioclostridium | ATGTCCAAGCACTGCGGA | ACAGTGCGCATTTCCAGGA | 59.25 | 59.93 | 187 | 49 |
100.00%
|
100.00%
|
| Primer 1260 | Massilioclostridium | TTTTGCCCAACCAGCCCA | AGGCGATGATGCAGCGTT | 60.04 | 60.12 | 173 | 49 |
100.00%
|
100.00%
|