| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Massilioclostridium | GCGGAAGTGTGCCCAAAT | TCCCCTGCACCAAAAGCTT | 58.63 | 59.77 | 165 | 116 |
100.00%
|
100.00%
|
| Primer 12 | Massilioclostridium | GCGGAAGTGTGCCCAAAT | CCCTGCACCAAAAGCTTCG | 58.63 | 59.71 | 163 | 115 |
100.00%
|
100.00%
|
| Primer 13 | Massilioclostridium | AGGACATTGGCGTTCCAGG | TCCTGTTCCCTTGCTGTCC | 60.00 | 59.24 | 182 | 114 |
100.00%
|
100.00%
|
| Primer 14 | Massilioclostridium | ACCGGACGTGCTTTACAGG | TTCTCACCGGAAGCCACTG | 60.01 | 59.63 | 299 | 112 |
100.00%
|
100.00%
|
| Primer 15 | Massilioclostridium | GCGGAAGTGTGCCCAAATC | CCAAAAGCTTCGGTTCCCA | 59.79 | 58.27 | 156 | 112 |
100.00%
|
100.00%
|
| Primer 16 | Massilioclostridium | TAGCTGCCTTAAGCCGTGG | ACAGCGTCAGCCTTTCCTG | 59.78 | 60.30 | 207 | 112 |
100.00%
|
100.00%
|
| Primer 17 | Massilioclostridium | AGGACATTGGCGTTCCAGG | TGCTGTCCGCATTTGTTCC | 60.00 | 59.04 | 171 | 109 |
100.00%
|
100.00%
|
| Primer 18 | Massilioclostridium | TGTGTCTTCCGCTGCTACC | ACCGTACCATCGCAACAGG | 59.71 | 60.08 | 198 | 109 |
100.00%
|
100.00%
|
| Primer 19 | Massilioclostridium | AGGACATTGGCGTTCCAGG | TTCCTGTTCCCTTGCTGTCC | 60.00 | 59.89 | 183 | 105 |
100.00%
|
100.00%
|
| Primer 20 | Massilioclostridium | TGCACCACCAGGGAAATGG | AGCCAGGAACCCCATTTCC | 60.23 | 59.61 | 209 | 105 |
100.00%
|
100.00%
|