| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 401 | Massilioclostridium | ACCCAAAAGCATTGCGGAAC | CCACTGACAGAATCTGCGGA | 59.97 | 59.75 | 168 | 63 |
100.00%
|
100.00%
|
| Primer 402 | Massilioclostridium | TCCCATTCCTGTTTGGCAGT | CCACTGACAGAATCTGCGGA | 59.52 | 59.75 | 223 | 63 |
100.00%
|
100.00%
|
| Primer 403 | Massilioclostridium | TGCTCCAGATGGACGGGAT | ACCACCATCTGCCTTTCCC | 60.38 | 59.62 | 214 | 63 |
100.00%
|
100.00%
|
| Primer 404 | Massilioclostridium | ATGCTCCAGATGGACGGGA | ACCACCATCTGCCTTTCCC | 60.38 | 59.62 | 215 | 63 |
100.00%
|
100.00%
|
| Primer 405 | Massilioclostridium | CCGTACAAACCGCAATGGA | TGGAGCAACATCGCCAGT | 58.45 | 59.25 | 152 | 63 |
100.00%
|
100.00%
|
| Primer 406 | Massilioclostridium | TGCTCCAACTCAGAGGGGA | TGGAGCAACACATCCGCAA | 59.84 | 60.23 | 159 | 62 |
100.00%
|
100.00%
|
| Primer 407 | Massilioclostridium | TGACACCATTGTGCAGGGA | GGGGTTGAATCCGTATTCCCA | 59.16 | 59.79 | 299 | 62 |
100.00%
|
100.00%
|
| Primer 408 | Massilioclostridium | TTGGGAAGGCGTTGACCA | CAGTTTGGCGTTTTCCGTGT | 59.08 | 59.90 | 217 | 62 |
100.00%
|
100.00%
|
| Primer 409 | Massilioclostridium | ATCCGGTGTTTTCCTGCGG | ACACCGCAATCCCAACAGG | 60.67 | 60.60 | 202 | 62 |
100.00%
|
100.00%
|
| Primer 410 | Massilioclostridium | TCAGCTGATTGACCTGCCG | TTGCCTGTTTGCCGTTGC | 60.08 | 59.89 | 218 | 62 |
100.00%
|
100.00%
|