| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Megasphaera | TCGATGATGCCGTTGCCA | ATCATGACCTGGGAGTGCG | 59.73 | 59.48 | 259 | 55 |
100.00%
|
66.67%
|
| Primer 82 | Megasphaera | GCCGTACCATCACGTCGTT | AGCGATTGTGCAGCCAGA | 60.44 | 59.65 | 176 | 55 |
100.00%
|
66.67%
|
| Primer 83 | Megasphaera | GTCTATGGCTTCGTCGGCA | ACGACGTGGGCAATCGTT | 59.86 | 59.97 | 224 | 53 |
100.00%
|
66.67%
|
| Primer 84 | Megasphaera | TGTCTATGGCTTCGTCGGC | ACGACGTGGGCAATCGTT | 59.86 | 59.97 | 225 | 53 |
100.00%
|
66.67%
|
| Primer 85 | Megasphaera | GGACGTCGCAAGTCATCCA | TGTTCGAAGCGGACGCAA | 60.08 | 60.28 | 235 | 53 |
100.00%
|
66.67%
|
| Primer 86 | Megasphaera | TCGATGATGCCGTTGCCA | GGCTGGTGGATCATGACCT | 59.73 | 59.08 | 268 | 53 |
100.00%
|
66.67%
|
| Primer 87 | Megasphaera | AGGACTTCGACAACGCCA | AGTGACCGCATTTGCCGA | 58.87 | 59.97 | 153 | 53 |
100.00%
|
66.67%
|
| Primer 88 | Megasphaera | AAACCGCTCCTGGCCAAA | CCCATTGGTTCGACGTGGA | 59.80 | 60.00 | 194 | 52 |
100.00%
|
66.67%
|
| Primer 89 | Megasphaera | ATTCCGTCGGCACACGTT | TCGTCGCGTTCAGGCATA | 59.97 | 59.12 | 173 | 52 |
100.00%
|
66.67%
|
| Primer 90 | Megasphaera | AAAGCAGACCGCATCGCT | CGGTTTTGCAGCAGCGAT | 60.05 | 59.44 | 168 | 51 |
100.00%
|
66.67%
|