| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Meiothermus | CGGCTATTGCTGTGGTTGC | TTTTCGCCACTCCTCCAGC | 59.86 | 60.30 | 240 | 132 |
100.00%
|
50.00%
|
| Primer 2 | Meiothermus | TACCGCTGCTGGAAGTTGG | TGGGGTTTGAGTGGCACTG | 60.00 | 60.15 | 247 | 130 |
100.00%
|
50.00%
|
| Primer 3 | Meiothermus | ACCCCAACGAATCAGCCTG | CGCAAAAGCAGAAGGCCTG | 60.00 | 60.08 | 154 | 130 |
100.00%
|
50.00%
|
| Primer 4 | Meiothermus | CCTGCTTCAGCGCATTACC | ATACAGCAAGCAGGCCTGG | 59.28 | 60.08 | 178 | 127 |
100.00%
|
50.00%
|
| Primer 5 | Meiothermus | TCGCGACTCCAAAACCCTC | AATCGGTGAGCAAGGCCTC | 60.01 | 60.08 | 197 | 124 |
100.00%
|
50.00%
|
| Primer 6 | Meiothermus | ACGGATTGATGCCCTGGTG | TTGCCTCGCAAACCCGAT | 60.08 | 59.97 | 201 | 123 |
100.00%
|
50.00%
|
| Primer 7 | Meiothermus | TTCGGACGGATTGATGCCC | TTGCCTCGCAAACCCGAT | 60.15 | 59.97 | 206 | 122 |
100.00%
|
50.00%
|
| Primer 8 | Meiothermus | AAGGTACAGGCCTTGCTGG | AGTCCAGGTGTTGACCAGC | 59.62 | 59.55 | 153 | 121 |
100.00%
|
50.00%
|
| Primer 9 | Meiothermus | TGGTCATCGTCAACCGCA | CACGAAGATGGCGTTGTGC | 59.26 | 60.15 | 296 | 120 |
100.00%
|
50.00%
|
| Primer 10 | Meiothermus | TCCCCAAACTTCAGCAGCA | AAGTGCGATGCCCACCAT | 59.46 | 59.64 | 155 | 120 |
100.00%
|
50.00%
|