| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Metamycoplasma | CGTATTCCCTCTTGCAGATGC | TTGGGCTGAAACTTCGTCAG | 59.13 | 58.41 | 199 | 146 |
100.00%
|
66.67%
|
| Primer 2 | Metamycoplasma | CGTATTCCCTCTTGCAGATGC | TGGGCTGAAACTTCGTCAGT | 59.13 | 59.53 | 198 | 132 |
100.00%
|
66.67%
|
| Primer 3 | Metamycoplasma | CGTATTCCCTCTTGCAGATGC | TTGGGCTGAAACTTCGTCAGT | 59.13 | 60.13 | 199 | 132 |
100.00%
|
66.67%
|
| Primer 4 | Metamycoplasma | CGTATTCCCTCTTGCAGATGC | GGGCTGAAACTTCGTCAGTTG | 59.13 | 59.74 | 197 | 131 |
100.00%
|
66.67%
|
| Primer 5 | Metamycoplasma | ACTTCAGTCGTAGCTGCGG | TGGGCTCTTCTTTAGGCTCA | 59.79 | 58.34 | 211 | 114 |
100.00%
|
33.33%
|
| Primer 6 | Metamycoplasma | ACTTCAGTCGTAGCTGCGG | TGGGCTCTTCTTTAGGCTCA | 59.79 | 58.34 | 196 | 114 |
100.00%
|
33.33%
|
| Primer 7 | Metamycoplasma | ACTTCAGTCGTAGCTGCGG | TTGGGCTCTTCTTTAGGCTCA | 59.79 | 59.01 | 212 | 109 |
100.00%
|
33.33%
|
| Primer 8 | Metamycoplasma | TGCCAACGTTGACCAAGC | TTGCCAACGCCAGTTTGG | 58.88 | 59.19 | 282 | 102 |
100.00%
|
33.33%
|
| Primer 9 | Metamycoplasma | TTGCCAACGTTGACCAAGC | TTGCCAACGCCAGTTTGG | 59.56 | 59.19 | 283 | 102 |
100.00%
|
33.33%
|
| Primer 10 | Metamycoplasma | TGCCAACGTTGACCAAGC | TTTGCCAACGCCAGTTTGG | 58.88 | 59.86 | 283 | 97 |
100.00%
|
33.33%
|