| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 101 | Methanobrevibacter | AGCAAAGATGGCAGGCCT | ACCTGGGTCTCCATGTTGAAC | 59.23 | 59.93 | 195 | 100 |
100.00%
|
50.00%
|
| Primer 102 | Methanobrevibacter | CTTACAGTTCCCTGCGGAGT | TCCCAGAAACCAGCTGCAG | 59.39 | 59.93 | 179 | 99 |
100.00%
|
50.00%
|
| Primer 103 | Methanobrevibacter | TGCTGCATGTAGGCGTTGT | GCTGCAGCTACACCAGTTG | 60.30 | 59.13 | 273 | 98 |
100.00%
|
75.00%
|
| Primer 104 | Methanobrevibacter | TCCCTGAAGGCGTTGAAGT | CGGTCATTGGAAAGTGAGCA | 58.86 | 58.48 | 264 | 98 |
100.00%
|
100.00%
|
| Primer 105 | Methanobrevibacter | GCTGCTGCTGCTATGAGGT | TGGAGTTCCACCTTTAGGGC | 60.15 | 59.30 | 234 | 98 |
100.00%
|
75.00%
|
| Primer 106 | Methanobrevibacter | CCTCGTGAAGCGGATGTTCT | TCCCTCCCACGATTTGCA | 60.11 | 58.51 | 287 | 97 |
100.00%
|
75.00%
|
| Primer 107 | Methanobrevibacter | AGACAGAGACCCACAACATGT | ACCGCATGCAGCACATTTTAA | 58.95 | 59.73 | 229 | 97 |
100.00%
|
75.00%
|
| Primer 108 | Methanobrevibacter | TAGGAAACCCAACCTGCGG | GCTGCCTTAAGTGCATCTGC | 59.62 | 59.90 | 256 | 97 |
100.00%
|
50.00%
|
| Primer 109 | Methanobrevibacter | GGAGGCATTCCAATTGGTGC | AGGGCAGGGAGAAAGAGTTG | 59.82 | 59.30 | 186 | 97 |
100.00%
|
50.00%
|
| Primer 110 | Methanobrevibacter | TGGAAGAAGCTACTCCTGCTG | TCCCCTTCTCTTACAGGACCC | 59.45 | 60.27 | 151 | 96 |
100.00%
|
50.00%
|