| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 131 | Methanobrevibacter | GGCCAGAACCTCTTCAACAAG | CGGCACATGGACAGTCACT | 59.12 | 60.00 | 299 | 94 |
100.00%
|
50.00%
|
| Primer 132 | Methanobrevibacter | AGTAGCAGTCACCGGAGCA | CAAGTTCACCAGCACCTGC | 60.61 | 59.34 | 155 | 94 |
100.00%
|
50.00%
|
| Primer 133 | Methanobrevibacter | TCCCTGAAGGCGTTGAAGT | AACGGTCATTGGAAAGTGAGC | 58.86 | 59.12 | 266 | 93 |
100.00%
|
100.00%
|
| Primer 134 | Methanobrevibacter | CGGTTCGGCATCTGATCCAT | AGGTGCATTTGGCTCAACC | 60.25 | 58.65 | 268 | 93 |
100.00%
|
50.00%
|
| Primer 135 | Methanobrevibacter | CGGTTCGGCATCTGATCCAT | AAGGTGCATTTGGCTCAACC | 60.25 | 59.32 | 269 | 93 |
100.00%
|
50.00%
|
| Primer 136 | Methanobrevibacter | ACATGAGCGACCCACTTGG | GGCCATTTGTACTTCAGCCAT | 60.00 | 58.90 | 239 | 93 |
100.00%
|
50.00%
|
| Primer 137 | Methanobrevibacter | GTTGCAGGAATAGCAGCCAT | CCAGCTGGGGTCTAGAAATCC | 58.60 | 59.86 | 291 | 93 |
100.00%
|
50.00%
|
| Primer 138 | Methanobrevibacter | GGAGGCATTCCAATTGGTGC | CCAGAACACATAGGGCAGGG | 59.82 | 60.11 | 197 | 93 |
100.00%
|
50.00%
|
| Primer 139 | Methanobrevibacter | TCCTCGTGAAGCGGATGTT | TCCCTCCCACGATTTGCA | 59.03 | 58.51 | 288 | 92 |
100.00%
|
75.00%
|
| Primer 140 | Methanobrevibacter | CAAACCTTCGGTTGCGGAG | GCTGCCTTAAGTGCATCTGC | 59.43 | 59.90 | 179 | 92 |
100.00%
|
50.00%
|