| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Methanobrevibacter | GCCCACCTCGTTCTGATGT | GGAAACAGTTGCAGGACCCA | 59.70 | 60.47 | 224 | 79 |
100.00%
|
75.00%
|
| Primer 222 | Methanobrevibacter | TAGTTGCAGGAATAGCAGCCA | CCAGCTGGGGTCTAGAAATCC | 59.44 | 59.86 | 293 | 79 |
100.00%
|
50.00%
|
| Primer 223 | Methanobrevibacter | TGATGAACCTGAACAAAGCCG | GCTGACAACCGCAACATTG | 59.12 | 58.20 | 179 | 79 |
100.00%
|
50.00%
|
| Primer 224 | Methanobrevibacter | AGCGGACACAGCAGGTACT | TTCACCTTCGGATGCGGA | 60.91 | 58.62 | 280 | 78 |
100.00%
|
75.00%
|
| Primer 225 | Methanobrevibacter | TGCTGCATGTAGGCGTTGT | CTGCAGCTACACCAGTTGG | 60.30 | 58.45 | 272 | 78 |
100.00%
|
75.00%
|
| Primer 226 | Methanobrevibacter | GTCCAACAGAAGAACCGCAG | TGCATTCTCCACCAGCACT | 59.13 | 59.24 | 185 | 78 |
100.00%
|
75.00%
|
| Primer 227 | Methanobrevibacter | ATGGAGACGTCCTAGGGGTA | AGCGTCAGTAGCTGGGTT | 58.77 | 58.21 | 181 | 78 |
100.00%
|
50.00%
|
| Primer 228 | Methanobrevibacter | AGCAAAGATGGCAGGCCT | ACCTGGGTCTCCATGTTGAA | 59.23 | 58.56 | 195 | 78 |
100.00%
|
50.00%
|
| Primer 229 | Methanobrevibacter | AGCAAAGATGGCAGGCCT | CCATACCTGGGTCTCCATGT | 59.23 | 58.49 | 199 | 78 |
100.00%
|
50.00%
|
| Primer 230 | Methanobrevibacter | AGCAAAGATGGCAGGCCT | CCTGGGTCTCCATGTTGAAC | 59.23 | 58.17 | 194 | 78 |
100.00%
|
50.00%
|