| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1011 | Methanosphaera | TGGGGATAGTGGTTGTGGAA | AGGCCAACGTCTCATCCAA | 58.25 | 58.93 | 259 | 72 |
100.00%
|
66.67%
|
| Primer 1012 | Methanosphaera | TTGGGGATAGTGGTTGTGGAA | AGGCCAACGTCTCATCCAA | 58.93 | 58.93 | 260 | 72 |
100.00%
|
66.67%
|
| Primer 1013 | Methanosphaera | AAAGGCGACATCACCGAGG | TGCTGCTCCTCTACCGGAA | 60.08 | 60.30 | 180 | 72 |
100.00%
|
66.67%
|
| Primer 1014 | Methanosphaera | GGCGACATCACCGAGGAAA | TGCTGCTCCTCTACCGGAA | 60.08 | 60.30 | 177 | 72 |
100.00%
|
66.67%
|
| Primer 1015 | Methanosphaera | GGGGAACACCACTCATGCT | CCCAGTATTTGTGCTGCTTCA | 59.62 | 58.84 | 202 | 72 |
100.00%
|
66.67%
|
| Primer 1016 | Methanosphaera | TCCTTCTGGTGGAGCTGGT | AACAGGATCAACAACAGCCC | 60.15 | 58.38 | 280 | 72 |
100.00%
|
66.67%
|
| Primer 1017 | Methanosphaera | TTTGGTGATGATGCAGCGG | TGTCCACCCACCATTGGT | 58.82 | 58.32 | 272 | 72 |
100.00%
|
66.67%
|
| Primer 1018 | Methanosphaera | CATGCATGTAACGGCAGGT | TGTGGTCCAGTTTGTCCCA | 58.52 | 58.69 | 204 | 72 |
100.00%
|
66.67%
|
| Primer 1019 | Methanosphaera | TCCGCATCTGTAGGTGCAG | TGCTGCTCCTGCACCAAT | 59.48 | 59.56 | 192 | 72 |
100.00%
|
66.67%
|
| Primer 1020 | Methanosphaera | TCGCATCTTCCGCATCTGT | TGCTGCTCCTGCACCAAT | 59.48 | 59.56 | 200 | 72 |
100.00%
|
66.67%
|