| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1101 | Methanosphaera | AGGGCAGGAAATTTCACAGC | GGCCAGTTCCACCATGCTT | 58.74 | 60.61 | 184 | 69 |
100.00%
|
66.67%
|
| Primer 1102 | Methanosphaera | TCCTTCTGGTGGAGCTGGT | ACAGGATCAACAACAGCCCTT | 60.15 | 59.85 | 279 | 69 |
100.00%
|
66.67%
|
| Primer 1103 | Methanosphaera | GATGCCCATGTACATGCAGA | ACACCATGTTTTGCCACACG | 58.03 | 59.90 | 182 | 69 |
100.00%
|
66.67%
|
| Primer 1104 | Methanosphaera | GGCCTGGACTTGCAGTAAGA | TGGCCATGGAATGTCTGGT | 59.68 | 58.91 | 260 | 69 |
100.00%
|
66.67%
|
| Primer 1105 | Methanosphaera | GACTCGGTCCTCAACTTGCA | AATGGTCCCCAAGCAACCA | 59.97 | 59.45 | 184 | 68 |
100.00%
|
33.33%
|
| Primer 1106 | Methanosphaera | TCACCCCAAGATGTAACAGCA | CTCCCTCATCAAGCTCTTCGT | 59.30 | 59.52 | 289 | 68 |
100.00%
|
33.33%
|
| Primer 1107 | Methanosphaera | TCACCCCAAGATGTAACAGCA | TCCCTCATCAAGCTCTTCGT | 59.30 | 58.44 | 288 | 68 |
100.00%
|
33.33%
|
| Primer 1108 | Methanosphaera | ACCCCAAGATGTAACAGCAGA | ACTCCCTCATCAAGCTCTTCG | 59.01 | 59.52 | 288 | 68 |
100.00%
|
33.33%
|
| Primer 1109 | Methanosphaera | CACCCCAAGATGTAACAGCAG | ACTCCCTCATCAAGCTCTTCG | 58.91 | 59.52 | 289 | 68 |
100.00%
|
33.33%
|
| Primer 1110 | Methanosphaera | TTAACGCACCTGACTGGGC | GGTCTTTGTTGCCACCGT | 60.30 | 58.16 | 171 | 68 |
100.00%
|
33.33%
|