| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 371 | Methanosphaera | ACAGGACCACTTCACTGGGA | GCTCCTGCTAATATACTGCCC | 60.40 | 58.02 | 158 | 96 |
100.00%
|
33.33%
|
| Primer 372 | Methanosphaera | CAGGACCACTTCACTGGGA | GCTCCTGCTAATATACTGCCC | 58.55 | 58.02 | 157 | 96 |
100.00%
|
33.33%
|
| Primer 373 | Methanosphaera | AGGAACAGGAACCAAGCCC | TGTGGAGGTGTTGAATGTCCC | 59.54 | 60.20 | 162 | 96 |
100.00%
|
33.33%
|
| Primer 374 | Methanosphaera | CATGGAGGAGCAGCTGGTAA | AGCTGCACCCCAGTAGAAA | 59.46 | 58.54 | 234 | 96 |
100.00%
|
33.33%
|
| Primer 375 | Methanosphaera | TGCAGGTGAATGGAGGGGA | TACCTCCAGGCCAACGTGT | 60.54 | 60.84 | 188 | 96 |
100.00%
|
66.67%
|
| Primer 376 | Methanosphaera | AATTGCACCAGTAACTGCAGT | AGGCGTGGTTGTGTTGGA | 58.41 | 59.41 | 261 | 96 |
100.00%
|
66.67%
|
| Primer 377 | Methanosphaera | AATTGCACCAGTAACTGCAGT | AAGGCGTGGTTGTGTTGGA | 58.41 | 60.08 | 262 | 96 |
100.00%
|
66.67%
|
| Primer 378 | Methanosphaera | TCCTGGACCAAGAGGAGGA | TGCTTCACAATCCCCTGGTC | 58.82 | 59.96 | 175 | 96 |
100.00%
|
66.67%
|
| Primer 379 | Methanosphaera | ACTTGTAGGCGTTGGTGGA | GTCATGGCAGTTCCTGAAACC | 58.86 | 59.46 | 273 | 96 |
100.00%
|
66.67%
|
| Primer 380 | Methanosphaera | TGGACGTGGTGGTGAAGAAG | GCACAAACTGGCGTGTCTG | 59.89 | 60.01 | 297 | 96 |
100.00%
|
66.67%
|