| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 471 | Methanosphaera | CCATGCATGTAACGGCAGG | TGTGGTCCAGTTTGTCCCAT | 59.27 | 59.15 | 205 | 93 |
100.00%
|
66.67%
|
| Primer 472 | Methanosphaera | CCATGCATGTAACGGCAGG | TTGTGGTCCAGTTTGTCCCAT | 59.27 | 59.78 | 206 | 93 |
100.00%
|
66.67%
|
| Primer 473 | Methanosphaera | ACCCAAGGGCATCAATTGACA | TGGGAATGATTGCAAGTGGC | 60.20 | 59.10 | 228 | 93 |
100.00%
|
66.67%
|
| Primer 474 | Methanosphaera | ACCCAAGGGCATCAATTGACA | GCATGTCTGCTTGTCCAGT | 60.20 | 58.06 | 210 | 93 |
100.00%
|
66.67%
|
| Primer 475 | Methanosphaera | ACCCAAGGGCATCAATTGACA | CTGGGAATGATTGCAAGTGGC | 60.20 | 60.13 | 229 | 93 |
100.00%
|
66.67%
|
| Primer 476 | Methanosphaera | ACAGGTGATGGTGATGCAACA | CCACTTGCATTGGTTGGGC | 60.20 | 60.00 | 265 | 93 |
100.00%
|
66.67%
|
| Primer 477 | Methanosphaera | ACGCGAATTTGGTGCACA | GCACTTTGAGCTCCACCAC | 58.57 | 59.05 | 225 | 93 |
100.00%
|
66.67%
|
| Primer 478 | Methanosphaera | TGCTGTGCATTTTCAACCAGG | AGCAAGAACCAGGGCTGT | 59.93 | 58.74 | 280 | 92 |
100.00%
|
33.33%
|
| Primer 479 | Methanosphaera | TGGCGTTACATGGAGAGGTG | AGTGGTACTGCTTGTCCTCTT | 59.75 | 58.67 | 258 | 92 |
100.00%
|
33.33%
|
| Primer 480 | Methanosphaera | GGCGTTACATGGAGAGGTGT | AGTGGTACTGCTTGTCCTCTT | 59.75 | 58.67 | 257 | 92 |
100.00%
|
33.33%
|